Cell, tissue, and organ culture: Coulter counter use in the enumeration of muscle and fat stem cells

Melinda Fernyhough-Culver, Deri L. Helterline-Icenoggle, Janet L. Vierck, Rodney Hill, Michael V. Dodson

Research output: Book chapter/Published conference paperChapter (peer-reviewed)

Abstract

Although the manual counting chamber (hemacytometer) is the gold standard for counting cells, this method is subject to great variability due to the “human factor.” The automated cell counter (Coulter Counter) can enumerate cells in less time and with greater accuracy than the hemacytometer by removing many of the steps in which errors are made. While the Coulter Counter (and others of its type) has been used for many years in the cell culture field, there have been few studies to validate its use with specific cell types. We conducted several experiments in which we assessed the accuracy of the Coulter Counter over counts made with a hemacytometer as well as validated its use for the counting of satellite cells and preadipocytes.
Original languageEnglish
Title of host publicationStem cells in aesthetic procedures
Subtitle of host publicationArt, science, and clinical techniques
EditorsMelvin A. Shiffman, Alberto Di Giuseppe, Franco Bassetto
Place of PublicationHeidleberg
PublisherSpringer
Chapter19
Pages 293-298
Number of pages6
Edition1st
ISBN (Electronic)9783642452079
ISBN (Print)9783642452062
DOIs
Publication statusPublished - 2014

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