Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride

Rui Yang, Yuqian Liu, Christopher Blanchard, Zhong Kai Zhou

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Phytoferritin cage has a nano-sized cavity to encapsulate bioactive molecules. In this work, a novel approach is presented that guanidine hydrochloride (GuHCl) (2mM) can expand the channel of apo-soybean seed ferritin (apoSSF) and promote the encapsulation of rutin molecules in apoSSF at pH 7.0 without the disassembly of the protein cage. Upon removal of GuHCl from SSF, a rutin encapsulation ratio of 10.1% was obtained; and the prepared rutin-loaded ferritin nanoparticles were homogeneously distributed, showing a shell-like morphology with a size of 12nm. By virtue of this interesting method, core molecules can be encapsulated within the ferritin cage in a benign condition without extreme pH changes, which is beneficial for the stability and bioactivity of the pH-sensitive molecules in food encapsulation and delivery of functional molecules.
Original languageEnglish
Pages (from-to)935-939
Number of pages5
JournalFood Chemistry
Early online dateJul 2017
Publication statusPublished - Feb 2018


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