Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride

Rui Yang, Yuqian Liu, Christopher Blanchard, Zhongkai Zhou

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Phytoferritin cage has a nano-sized cavity to encapsulate bioactive molecules. In this work, a novel approach is presented that guanidine hydrochloride (GuHCl) (2mM) can expand the channel of apo-soybean seed ferritin (apoSSF) and promote the encapsulation of rutin molecules in apoSSF at pH 7.0 without the disassembly of the protein cage. Upon removal of GuHCl from SSF, a rutin encapsulation ratio of 10.1% was obtained; and the prepared rutin-loaded ferritin nanoparticles were homogeneously distributed, showing a shell-like morphology with a size of 12nm. By virtue of this interesting method, core molecules can be encapsulated within the ferritin cage in a benign condition without extreme pH changes, which is beneficial for the stability and bioactivity of the pH-sensitive molecules in food encapsulation and delivery of functional molecules.
Original languageEnglish
Pages (from-to)935-939
Number of pages5
JournalFood Chemistry
Volume240
Early online dateJul 2017
DOIs
Publication statusPublished - Feb 2018

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guanidines
Rutin
Guanidine
ferritin
rutin
Ferritins
encapsulation
Encapsulation
cages
Molecules
Soybeans
Seeds
Seed
soybeans
nanoparticles
seeds
Nanoparticles
Bioactivity
Food
phytoferritin

Cite this

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title = "Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride",
abstract = "Phytoferritin cage has a nano-sized cavity to encapsulate bioactive molecules. In this work, a novel approach is presented that guanidine hydrochloride (GuHCl) (2mM) can expand the channel of apo-soybean seed ferritin (apoSSF) and promote the encapsulation of rutin molecules in apoSSF at pH 7.0 without the disassembly of the protein cage. Upon removal of GuHCl from SSF, a rutin encapsulation ratio of 10.1{\%} was obtained; and the prepared rutin-loaded ferritin nanoparticles were homogeneously distributed, showing a shell-like morphology with a size of 12nm. By virtue of this interesting method, core molecules can be encapsulated within the ferritin cage in a benign condition without extreme pH changes, which is beneficial for the stability and bioactivity of the pH-sensitive molecules in food encapsulation and delivery of functional molecules.",
keywords = "Nano-encapsulation, Guanidine hydrochloride, Protein channel, Ferritin cage",
author = "Rui Yang and Yuqian Liu and Christopher Blanchard and Zhongkai Zhou",
note = "Includes bibliographical references.",
year = "2018",
month = "2",
doi = "10.1016/j.foodchem.2017.07.088",
language = "English",
volume = "240",
pages = "935--939",
journal = "Journal of Micronutrient Analysis",
issn = "0308-8146",
publisher = "Elsevier BV",

}

Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride. / Yang, Rui; Liu, Yuqian; Blanchard, Christopher; Zhou, Zhongkai.

In: Food Chemistry, Vol. 240, 02.2018, p. 935-939.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride

AU - Yang, Rui

AU - Liu, Yuqian

AU - Blanchard, Christopher

AU - Zhou, Zhongkai

N1 - Includes bibliographical references.

PY - 2018/2

Y1 - 2018/2

N2 - Phytoferritin cage has a nano-sized cavity to encapsulate bioactive molecules. In this work, a novel approach is presented that guanidine hydrochloride (GuHCl) (2mM) can expand the channel of apo-soybean seed ferritin (apoSSF) and promote the encapsulation of rutin molecules in apoSSF at pH 7.0 without the disassembly of the protein cage. Upon removal of GuHCl from SSF, a rutin encapsulation ratio of 10.1% was obtained; and the prepared rutin-loaded ferritin nanoparticles were homogeneously distributed, showing a shell-like morphology with a size of 12nm. By virtue of this interesting method, core molecules can be encapsulated within the ferritin cage in a benign condition without extreme pH changes, which is beneficial for the stability and bioactivity of the pH-sensitive molecules in food encapsulation and delivery of functional molecules.

AB - Phytoferritin cage has a nano-sized cavity to encapsulate bioactive molecules. In this work, a novel approach is presented that guanidine hydrochloride (GuHCl) (2mM) can expand the channel of apo-soybean seed ferritin (apoSSF) and promote the encapsulation of rutin molecules in apoSSF at pH 7.0 without the disassembly of the protein cage. Upon removal of GuHCl from SSF, a rutin encapsulation ratio of 10.1% was obtained; and the prepared rutin-loaded ferritin nanoparticles were homogeneously distributed, showing a shell-like morphology with a size of 12nm. By virtue of this interesting method, core molecules can be encapsulated within the ferritin cage in a benign condition without extreme pH changes, which is beneficial for the stability and bioactivity of the pH-sensitive molecules in food encapsulation and delivery of functional molecules.

KW - Nano-encapsulation

KW - Guanidine hydrochloride

KW - Protein channel

KW - Ferritin cage

U2 - 10.1016/j.foodchem.2017.07.088

DO - 10.1016/j.foodchem.2017.07.088

M3 - Article

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VL - 240

SP - 935

EP - 939

JO - Journal of Micronutrient Analysis

JF - Journal of Micronutrient Analysis

SN - 0308-8146

ER -