Characterisation and functional properties of Australian rice protein isolates

Samson Agboola, Darren Ng, Dominic Mills

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Albumin, globulin, glutelin and prolamin fractions were isolated from an Australian rice variety (cv. Langi) and characterised by yield, protein content and molecular weight profile using both capillary electrophoresis (SDS-CE) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The influence of pre-extraction enzymatic hydrolysis of starch and heating to 70 °C was also investigated, as was the extraction of the glutelin fraction without prior removal of the albumin and globulin fractions. Pre-extraction treatment affected mainly the albumin fraction, increasing dry matter yield but reducing protein content. SDS-CE was able to separate the protein fractions over a wider molecular weight range than SDS-PAGE, and the peaks from SDS-CE showed slightly higher molecular weight compared to equivalent bands from SDS-PAGE. The glutelin fraction extracted without prior removal of albumin and globulin fractions had different characteristics compared to those obtained by conventional extraction methods. Pre-extraction hydrolysis of starch did not significantly affect the emulsifying, foaming and gelling properties of extracted protein. Although rice glutelin had poor solubility, emulsifying and foaming properties in aqueous systems, it had good gelling properties which could be important for food applications.
Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalJournal of Cereal Science
Volume41
Issue number3
DOIs
Publication statusPublished - 2005

Fingerprint

rice protein
Glutens
protein isolates
glutelins
functional properties
Albumins
Globulins
Sodium Dodecyl Sulfate
albumins
Polyacrylamide Gel Electrophoresis
Electrophoresis
globulins
Molecular Weight
gelling properties
polyacrylamide gel electrophoresis
foaming properties
Starch
emulsifying properties
Molecular weight
molecular weight

Cite this

Agboola, Samson ; Ng, Darren ; Mills, Dominic. / Characterisation and functional properties of Australian rice protein isolates. In: Journal of Cereal Science. 2005 ; Vol. 41, No. 3. pp. 283-290.
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Characterisation and functional properties of Australian rice protein isolates. / Agboola, Samson; Ng, Darren; Mills, Dominic.

In: Journal of Cereal Science, Vol. 41, No. 3, 2005, p. 283-290.

Research output: Contribution to journalArticle

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T1 - Characterisation and functional properties of Australian rice protein isolates

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AU - Ng, Darren

AU - Mills, Dominic

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