Characterisation, bioaccessibility and antioxidant activity of polyphenols from pigmented wheat and barley

Research output: Other contribution to conferencePresentation only


Pigmented cereals are believed to be a source of bioaccessible polyphenols which enhance health outcomes through their antioxidant properties. This study aimed to characterise polyphenols from Hordeum vulgare (purple barley), Triticum turgidum (purple wheat) and Triticum aestivum (blue wheat) to evaluate their bioaccessibility and antioxidant activity. Methanol extracts of cereals were used as a control to identify individual polyphenols and quantify their relative antioxidant levels using a UHPLC/MS-Online ABTS•+ system. Digestion of the cereal flour was performed using fluids containing electrolytes, digestive enzymes, and pH alterations to mimic upper gastrointestinal digestion. Using benchtop assays such involving Folin-Ciocalteu, FRAP, DPPH and ABTS•+, the bioaccessibility and antioxidant activity of polyphenols in both the gastric and intestinal phases were determined. Between cereals, the bioaccessible phenolic content was similar following intestinal digestion, but the antioxidant activity was significantly different (purple barley > purple wheat > blue wheat; p < 0.01). Among the polyphenols identified, flavan-3-ols and anthocyanins were the least bioaccessible whereas flavones were the most bioaccessible after digestion. This study demonstrated that these pigmented cereal varieties are sources of bioaccessible polyphenols with antioxidant activity, hence, promoting the value of these grains as potential functional food products.
Original languageEnglish
Publication statusPublished - 24 Aug 2022
EventAustralian Grain Science Association Conference 2022: AGSA - Rydges Capital Hill, Canberra, Australia
Duration: 24 Aug 202226 Aug 2022
Conference number: 72 (Conference program)


ConferenceAustralian Grain Science Association Conference 2022
Abbreviated titleGrains: Many Opportunities
OtherOn behalf of the conference organising committee, it is a pleasure to welcome you to join us in Canberra for the 72nd annual conference from 24th to 26th August 2022, COVID permitting.

The conference will be held in the Rydges Capital Hill in Forrest, a 10-minute drive from Canberra airport, close to Parliament House and an easy walk to Manuka shops which has many dining options. Our conference provides a forum for scientists and the grains industry to hear and discuss the latest innovations in science.

A diverse program covering all grain types, end-uses and markets is being developed. Updates will be continually added to the conference web-page so stay tuned for the call for papers on the 1st of May, 2022.

We strongly encourage student participation, and students are encouraged to apply for the AGSA Travel Awards.

For potential sponsors and exhibitors, a range of packages are available to increase your exposure. For further details, please click here or contact Dr Joe Panozzo.
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