Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum

P.R. Boag, J.C. Parsons, P.J.A. Presidente, T.W. Spithill, J.L. Sexton

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Infection with Ancylostoma caninum, an intestinal hookworm of dogs, can cause debilitating and potentially life-threatening disease. In the current study, protective immunity to hookworm infection was induced in dogs following vaccination with irradiation-attenuated third-stage larvae (L3) with significant reductions in both worm (P<0.03) and faecal egg counts (P<0.0004) following a challenge infection. Vaccination with irradiated L3 and challenge with infective L3 stimulated a dominant antibody response to antigens of less than 20kDa in an excretory/secretory extract from adult parasites. Immunoscreening of an adult A. caninum cDNA library with antisera from the vaccine trial identified a number of clones. The three clones with the strongest immunoreactivity proved to be identical and encoded a peptide with similarity to the N-terminal domain of the tissue matrix metalloproteinase inhibitor (TIMP)-2 mammalian tissue metalloproteinase inhibitor family.
Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume92
Issue number1-2
DOIs
Publication statusPublished - 2003

Fingerprint

Dive into the research topics of 'Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum'. Together they form a unique fingerprint.

Cite this