Abstract
protrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.
| Original language | English |
|---|---|
| Article number | doi:10.1016/j.rvsc.2004.01.006 |
| Pages (from-to) | 131-141 |
| Number of pages | 12 |
| Journal | Research in Veterinary Science |
| Volume | 77 |
| Issue number | 2 |
| Publication status | Published - 2004 |
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Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. / Loukopoulos, Panayiotis; O'Brien, T.; Ghoddusi, Majid; Mungall, Bruce A.; Robinson, Wayne F.
In: Research in Veterinary Science, Vol. 77, No. 2, doi:10.1016/j.rvsc.2004.01.006, 2004, p. 131-141.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.
AU - Loukopoulos, Panayiotis
AU - O'Brien, T.
AU - Ghoddusi, Majid
AU - Mungall, Bruce A.
AU - Robinson, Wayne F.
PY - 2004
Y1 - 2004
N2 - Three canine osteosarcoma cell lines were established from spontaneous pelvic and radial osteosarcomas. The cell populations cultured exhibited characteristics of malignancy and consisted of adherent, pleomorphic, mostly large spindle-shaped or polyhedral cells, characterised by the presence of numerous cytoplasmic granules and vacuoles. The main ultrastructural features included the presence of abundant rough endoplasmic reticulum and numerous cytoplasmic vesicles, deposit vacuoles and small cytoplasmicprotrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.
AB - Three canine osteosarcoma cell lines were established from spontaneous pelvic and radial osteosarcomas. The cell populations cultured exhibited characteristics of malignancy and consisted of adherent, pleomorphic, mostly large spindle-shaped or polyhedral cells, characterised by the presence of numerous cytoplasmic granules and vacuoles. The main ultrastructural features included the presence of abundant rough endoplasmic reticulum and numerous cytoplasmic vesicles, deposit vacuoles and small cytoplasmicprotrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.
KW - comparative oncology
KW - Comparative pathology
KW - in vitro models
KW - osteosarcoma
KW - bone tumours
KW - childhood diseases
UR - https://www.ncbi.nlm.nih.gov/pubmed/15196903
M3 - Article
VL - 77
SP - 131
EP - 141
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
M1 - doi:10.1016/j.rvsc.2004.01.006
ER -