Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.

Panayiotis Loukopoulos, T. O'Brien, Majid Ghoddusi, Bruce A. Mungall, Wayne F. Robinson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Three canine osteosarcoma cell lines were established from spontaneous pelvic and radial osteosarcomas. The cell populations cultured exhibited characteristics of malignancy and consisted of adherent, pleomorphic, mostly large spindle-shaped or polyhedral cells, characterised by the presence of numerous cytoplasmic granules and vacuoles. The main ultrastructural features included the presence of abundant rough endoplasmic reticulum and numerous cytoplasmic vesicles, deposit vacuoles and small cytoplasmic
protrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.
Original languageEnglish
Article numberdoi:10.1016/j.rvsc.2004.01.006
Pages (from-to)131-141
Number of pages12
JournalResearch in Veterinary Science
Volume77
Issue number2
Publication statusPublished - 2004

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osteosarcoma
metalloproteinases
Osteosarcoma
Matrix Metalloproteinases
Canidae
cell lines
Cell Line
gelatinase A
gelatinase B
dogs
Vacuoles
vacuoles
Cytoplasmic Vesicles
osteopontin
cytoplasmic granules
Cytoplasmic Granules
factor VIII
Retinoblastoma Protein
desmin
Osteopontin

Cite this

Loukopoulos, P., O'Brien, T., Ghoddusi, M., Mungall, B. A., & Robinson, W. F. (2004). Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. Research in Veterinary Science, 77(2), 131-141. [doi:10.1016/j.rvsc.2004.01.006].
Loukopoulos, Panayiotis ; O'Brien, T. ; Ghoddusi, Majid ; Mungall, Bruce A. ; Robinson, Wayne F. / Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. In: Research in Veterinary Science. 2004 ; Vol. 77, No. 2. pp. 131-141.
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abstract = "Three canine osteosarcoma cell lines were established from spontaneous pelvic and radial osteosarcomas. The cell populations cultured exhibited characteristics of malignancy and consisted of adherent, pleomorphic, mostly large spindle-shaped or polyhedral cells, characterised by the presence of numerous cytoplasmic granules and vacuoles. The main ultrastructural features included the presence of abundant rough endoplasmic reticulum and numerous cytoplasmic vesicles, deposit vacuoles and small cytoplasmicprotrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.",
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Loukopoulos, P, O'Brien, T, Ghoddusi, M, Mungall, BA & Robinson, WF 2004, 'Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases.', Research in Veterinary Science, vol. 77, no. 2, doi:10.1016/j.rvsc.2004.01.006, pp. 131-141.

Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. / Loukopoulos, Panayiotis; O'Brien, T.; Ghoddusi, Majid; Mungall, Bruce A.; Robinson, Wayne F.

In: Research in Veterinary Science, Vol. 77, No. 2, doi:10.1016/j.rvsc.2004.01.006, 2004, p. 131-141.

Research output: Contribution to journalArticle

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AB - Three canine osteosarcoma cell lines were established from spontaneous pelvic and radial osteosarcomas. The cell populations cultured exhibited characteristics of malignancy and consisted of adherent, pleomorphic, mostly large spindle-shaped or polyhedral cells, characterised by the presence of numerous cytoplasmic granules and vacuoles. The main ultrastructural features included the presence of abundant rough endoplasmic reticulum and numerous cytoplasmic vesicles, deposit vacuoles and small cytoplasmicprotrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.

KW - comparative oncology

KW - Comparative pathology

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KW - bone tumours

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Loukopoulos P, O'Brien T, Ghoddusi M, Mungall BA, Robinson WF. Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. Research in Veterinary Science. 2004;77(2):131-141. doi:10.1016/j.rvsc.2004.01.006.