protrusions. Zymography showed that the cell lines produce high levels of MMP-2 and MMP-9, enzymes directly involved in crucial aspects of the metastatic process. Consistent with their osteoblastic lineage and malignant phenotype, all cell lines were immunoreactive to vimentin, osteopontin, PCNA, p53, MMP-2 and MMP-9, while they were negative for cytokeratin, desmin, SMA, Factor VIII, NSE, GFAP, Rb and p21 protein. No retroviral particles or RNA were detected ultrastructurally or with RT-PCR, although the possibility of viral involvement in osteosarcoma cannot be excluded. The new cell lines provide excellent in vitro models that may allow further studies on the pathobiology of canine osteosarcoma to be undertaken.
|Number of pages||12|
|Journal||Research in Veterinary Science|
|Publication status||Published - 2004|
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Loukopoulos, P. (Recipient), Robinson, W. F. (Recipient), Thornton, J. R. (Recipient) & Straw, R. C. (Recipient), 1998
Loukopoulos, P. (Recipient), Straw, R. C. (Recipient), Thornton, J. R. (Recipient) & Robinson, W. F. (Recipient), 1999
Bequest of Brown Emily Perrett “for the benefit of animal health and welfare, including diagnosis and treatment of cancer in cats and dogs.”
Loukopoulos, P. (Recipient), Robinson, W. F. (Recipient) & Thornton, J. R. (Recipient), 1997