Characterization of antioxidant active phenolic compounds in sorghum grains using uhplc-online ABTS and LC/MS QTOF

Shiwangni Rao, Abishek Santhakumar, Kenneth Chinkwo, Gangcheng Wu, Stuart Johnson, Christopher Blanchard

Research output: Other contribution to conferenceAbstract


Phenolic composition and relative antioxidant activity were investigated in six varieties of sorghum including pigmented and non-pigmented pericarp varieties. Whole grain sorghum extracts were subjected to acidified acetone extraction and were analysed using UHPLC with an attached online ABTS system and Q-TOF LC/MS. Results showed that the black pericarp variety Shawaya short black 1 and the brown pericarp IS11316 had the highest total phenolic content (TPC) and total proanthocyanidin content (TPAC), consequently resulting in overall high antioxidant activity. The phenolic compounds that had the highest antioxidant activity in Shawaya and IS11316 were catechin, 1-O-cafferolglycerol-O-glucoside, taxifolin and pentahydroxyflavanone-(3->4)-catechin-7-O-glucoside. Furthermore, comparison of the phenolic profiles has shown that the varieties with higher antioxidant activity generally had higher quantities and more diverse range of polyphenols. Thus this study indicates that there is no single compound in sorghum that results in its high antioxidant activity but rather it is the cumulative effect of various phenolic compounds.
Original languageEnglish
Number of pages1
Publication statusPublished - 12 Sept 2018
Event68th Australasian Grain Science Conference - Charles Sturt University, Wagga Wagga, Australia
Duration: 10 Sept 201813 Sept 2018 (Conference booklet)


Conference68th Australasian Grain Science Conference
Abbreviated titleGrain Science Transforming our Future
CityWagga Wagga
Internet address


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