Characterization of bacterial communities associated with the Tyrian purple producing gland in a marine gastropod

Ajit Kumar Ngangbam, Abdul Baten, Daniel L.E. Waters, Steve Whalan, Kirsten Benkendorff

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Dicathais obita is a marine mollusc recognised for the production of anticancer compounds that are precursors to Tyrian purple. This study aimed to assess the diversity and identity of bacteria associated with the Tyrian purple producing hypobranchial gland, in comparison with foot tissue, using a high-throughput sequencing approach. Taxonomic and phylogenetic analysis of variable region V1-V3 of 16S rRNA bacterial gene amplicons in QIIME and MEGAN were carried out. This analysis revealed a highly diverse bacterial assemblage associated with the hypobranchial gland and foot tissues of D. orbita. The dominant bacterial phylum in the 16S rRNA bacterial profiling data set was Proteobacteria followed by Bacteroidetes, Tenericutes and Spirochaetes. In comparison to the foot, the hypobranchial gland had significantly lower bacterial diversity and a different community composition, based on taxonomic assignment at the genus level. A higher abundance of indole producing Vibrio spp. and the presence of bacteria with brominating capabilities in the hypobranchial gland suggest bacteria have a potential role in biosynthesis of Tyrian purple in D. orbita.

Original languageEnglish
Article numbere0140725
Pages (from-to)1-19
Number of pages19
JournalPLoS One
Volume10
Issue number10
DOIs
Publication statusPublished - 21 Oct 2015

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