Characterization of fecal fat composition and gut derived fecal microbiota in high-fat diet fed rats following intervention with chito-oligosaccharide and resistant starch complexes

Wenting Shang, Xu Si, Zhongkai Zhou, Li Ying, Padraig Strappe, Christopher Blanchard

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The current study analyzed the different effects of intervention in high-fat diet fed rats using chito-oligosaccharides (CO group), resistant starch (RS group) and their complexes (CO-RS group), respectively. Properties such as fecal fat composition, gut derived fecal microbiota and fecal metabolites were investigated. The results show that supplementation with CO-RS in the high-fat diet led to the highest level of fecal fat excretion, followed by the CO and RS groups, and its fatty acid composition was characterized by a lower ∑UFA and higher ∑SFA levels. Furthermore, CO-RS consumption significantly enhanced the excretion of bile acids in the feces, which might be associated with a higher conversion of cholesterol into bile acids and the enhanced binding capacity with the bile acids. The fecal microbiota profile using amplified V4 rDNA suggested that rats in the CO-RS group developed an increased richness and diversity in the gut bacterial community compared to the high-fat diet group. More importantly, the CO-RS intake significantly increased the abundance of both Lactobacillus and Bifidobacterium, indicating their potential use as prebiotics. Furthermore, the highest abundance of Allobaculum and Blautia genus in the feces of the CO-RS group was also found in this study, which is highly related to the highest production of short-chain fatty acids (SCFAs) in the colon among all the animals groups. A histological analysis of colon tissue revealed that the CO-RS supplemented diet was associated with a greater degree of thickness of the mucosa layer compared to the rats in the untreated high-fat diet group. The resulting shift in the gut microbiome, increased metabolite (SCFAs) production and increased thickness of the mucosal layer may provide profound influences of CO-RS consumption on gut protection.
Original languageEnglish
Pages (from-to)4374-4383
Number of pages10
JournalFood and Function
Volume8
Issue number12
DOIs
Publication statusPublished - 2017

Fingerprint

chitooligosaccharides
resistant starch
Microbiota
High Fat Diet
Carbon Monoxide
high fat diet
Oligosaccharides
Starch
bile acids
digestive system
Fats
microorganisms
rats
short chain fatty acids
lipids
colon
excretion
feces
metabolites
Bile Acids and Salts

Cite this

@article{0e05ad4f9aaf4bc8b587081d28373e04,
title = "Characterization of fecal fat composition and gut derived fecal microbiota in high-fat diet fed rats following intervention with chito-oligosaccharide and resistant starch complexes",
abstract = "The current study analyzed the different effects of intervention in high-fat diet fed rats using chito-oligosaccharides (CO group), resistant starch (RS group) and their complexes (CO-RS group), respectively. Properties such as fecal fat composition, gut derived fecal microbiota and fecal metabolites were investigated. The results show that supplementation with CO-RS in the high-fat diet led to the highest level of fecal fat excretion, followed by the CO and RS groups, and its fatty acid composition was characterized by a lower ∑UFA and higher ∑SFA levels. Furthermore, CO-RS consumption significantly enhanced the excretion of bile acids in the feces, which might be associated with a higher conversion of cholesterol into bile acids and the enhanced binding capacity with the bile acids. The fecal microbiota profile using amplified V4 rDNA suggested that rats in the CO-RS group developed an increased richness and diversity in the gut bacterial community compared to the high-fat diet group. More importantly, the CO-RS intake significantly increased the abundance of both Lactobacillus and Bifidobacterium, indicating their potential use as prebiotics. Furthermore, the highest abundance of Allobaculum and Blautia genus in the feces of the CO-RS group was also found in this study, which is highly related to the highest production of short-chain fatty acids (SCFAs) in the colon among all the animals groups. A histological analysis of colon tissue revealed that the CO-RS supplemented diet was associated with a greater degree of thickness of the mucosa layer compared to the rats in the untreated high-fat diet group. The resulting shift in the gut microbiome, increased metabolite (SCFAs) production and increased thickness of the mucosal layer may provide profound influences of CO-RS consumption on gut protection.",
author = "Wenting Shang and Xu Si and Zhongkai Zhou and Li Ying and Padraig Strappe and Christopher Blanchard",
year = "2017",
doi = "10.1039/c7fo01244f",
language = "English",
volume = "8",
pages = "4374--4383",
journal = "Food and Function",
issn = "2042-6496",
publisher = "Royal Society of Chemistry",
number = "12",

}

Characterization of fecal fat composition and gut derived fecal microbiota in high-fat diet fed rats following intervention with chito-oligosaccharide and resistant starch complexes. / Shang, Wenting; Si, Xu; Zhou, Zhongkai; Ying, Li; Strappe, Padraig; Blanchard, Christopher.

In: Food and Function, Vol. 8, No. 12, 2017, p. 4374-4383.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of fecal fat composition and gut derived fecal microbiota in high-fat diet fed rats following intervention with chito-oligosaccharide and resistant starch complexes

AU - Shang, Wenting

AU - Si, Xu

AU - Zhou, Zhongkai

AU - Ying, Li

AU - Strappe, Padraig

AU - Blanchard, Christopher

PY - 2017

Y1 - 2017

N2 - The current study analyzed the different effects of intervention in high-fat diet fed rats using chito-oligosaccharides (CO group), resistant starch (RS group) and their complexes (CO-RS group), respectively. Properties such as fecal fat composition, gut derived fecal microbiota and fecal metabolites were investigated. The results show that supplementation with CO-RS in the high-fat diet led to the highest level of fecal fat excretion, followed by the CO and RS groups, and its fatty acid composition was characterized by a lower ∑UFA and higher ∑SFA levels. Furthermore, CO-RS consumption significantly enhanced the excretion of bile acids in the feces, which might be associated with a higher conversion of cholesterol into bile acids and the enhanced binding capacity with the bile acids. The fecal microbiota profile using amplified V4 rDNA suggested that rats in the CO-RS group developed an increased richness and diversity in the gut bacterial community compared to the high-fat diet group. More importantly, the CO-RS intake significantly increased the abundance of both Lactobacillus and Bifidobacterium, indicating their potential use as prebiotics. Furthermore, the highest abundance of Allobaculum and Blautia genus in the feces of the CO-RS group was also found in this study, which is highly related to the highest production of short-chain fatty acids (SCFAs) in the colon among all the animals groups. A histological analysis of colon tissue revealed that the CO-RS supplemented diet was associated with a greater degree of thickness of the mucosa layer compared to the rats in the untreated high-fat diet group. The resulting shift in the gut microbiome, increased metabolite (SCFAs) production and increased thickness of the mucosal layer may provide profound influences of CO-RS consumption on gut protection.

AB - The current study analyzed the different effects of intervention in high-fat diet fed rats using chito-oligosaccharides (CO group), resistant starch (RS group) and their complexes (CO-RS group), respectively. Properties such as fecal fat composition, gut derived fecal microbiota and fecal metabolites were investigated. The results show that supplementation with CO-RS in the high-fat diet led to the highest level of fecal fat excretion, followed by the CO and RS groups, and its fatty acid composition was characterized by a lower ∑UFA and higher ∑SFA levels. Furthermore, CO-RS consumption significantly enhanced the excretion of bile acids in the feces, which might be associated with a higher conversion of cholesterol into bile acids and the enhanced binding capacity with the bile acids. The fecal microbiota profile using amplified V4 rDNA suggested that rats in the CO-RS group developed an increased richness and diversity in the gut bacterial community compared to the high-fat diet group. More importantly, the CO-RS intake significantly increased the abundance of both Lactobacillus and Bifidobacterium, indicating their potential use as prebiotics. Furthermore, the highest abundance of Allobaculum and Blautia genus in the feces of the CO-RS group was also found in this study, which is highly related to the highest production of short-chain fatty acids (SCFAs) in the colon among all the animals groups. A histological analysis of colon tissue revealed that the CO-RS supplemented diet was associated with a greater degree of thickness of the mucosa layer compared to the rats in the untreated high-fat diet group. The resulting shift in the gut microbiome, increased metabolite (SCFAs) production and increased thickness of the mucosal layer may provide profound influences of CO-RS consumption on gut protection.

U2 - 10.1039/c7fo01244f

DO - 10.1039/c7fo01244f

M3 - Article

VL - 8

SP - 4374

EP - 4383

JO - Food and Function

JF - Food and Function

SN - 2042-6496

IS - 12

ER -