Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells

Yan Yan Yi; Shuang Xiu Wan; Ya Xin Hou; Jia Cheng; Jian Hua Guo; Shaun Wang; Ajab Khan; Na Sun; Hongquan Li

doi:10.1016/j.ecoenv.2020.110401

Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells

Yan Yan Yi, Shuang Xiu Wan, Ya Xin Hou, Jia Cheng, Jian Hua Guo, Shaun Wang, Ajab Khan, Na Sun, Hongquan Li

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Abstract

Zearalenone (ZEA), a toxic substance produced by Fusarium fungi, accumulated in cereals grain and animal feed, causes injury to humans and animals. ZEA can induce obvious reproductive toxicity with the ovarian granulosa cells (GCs) as the main target. However, the study on exploring the protective compounds against ZEA-induced mouse primary ovarian GCs damage remains less. In the current study, the protective effect of 20 compounds derived from traditional Chinese medicines (TCMs) on the injury of mouse GCs caused by ZEA were evaluated using MTT assay and the cell morphology. Our results showed that chlorogenic acid (250, 500, and 1000 μg/mL) significantly suppress ZEA-induced GCs death. Western blot analysis suggested chlorogenic acid could rescue the up-regulated apoptosis of GCs induced by ZEA via attenuating the protein expression of cleaved caspase-3, the ratio of Bax/Bcl-2 and cleaved-PARP. Our results provide strong evidence that chlorogenic acid warrants further optimization for more potent and safer compounds for against the ZEA lead toxicity to humans and animals.

Original language	English
Article number	110401
Pages (from-to)	1-9
Number of pages	9
Journal	Ecotoxicology and Environmental Safety
Volume	194
Early online date	03 Mar 2020
DOIs	https://doi.org/10.1016/j.ecoenv.2020.110401
Publication status	Published - May 2020

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title = "Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells",

abstract = "Zearalenone (ZEA), a toxic substance produced by Fusarium fungi, accumulated in cereals grain and animal feed, causes injury to humans and animals. ZEA can induce obvious reproductive toxicity with the ovarian granulosa cells (GCs) as the main target. However, the study on exploring the protective compounds against ZEA-induced mouse primary ovarian GCs damage remains less. In the current study, the protective effect of 20 compounds derived from traditional Chinese medicines (TCMs) on the injury of mouse GCs caused by ZEA were evaluated using MTT assay and the cell morphology. Our results showed that chlorogenic acid (250, 500, and 1000 μg/mL) significantly suppress ZEA-induced GCs death. Western blot analysis suggested chlorogenic acid could rescue the up-regulated apoptosis of GCs induced by ZEA via attenuating the protein expression of cleaved caspase-3, the ratio of Bax/Bcl-2 and cleaved-PARP. Our results provide strong evidence that chlorogenic acid warrants further optimization for more potent and safer compounds for against the ZEA lead toxicity to humans and animals.",

keywords = "Apoptosis, Chlorogenic acid, Injury, Ovarian granulosa cells, Zearalenone",

author = "Yi, {Yan Yan} and Wan, {Shuang Xiu} and Hou, {Ya Xin} and Jia Cheng and Guo, {Jian Hua} and Shaun Wang and Ajab Khan and Na Sun and Hongquan Li",

note = "Includes bibliographical references",

year = "2020",

month = may,

doi = "10.1016/j.ecoenv.2020.110401",

language = "English",

volume = "194",

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T1 - Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells

AU - Yi, Yan Yan

AU - Wan, Shuang Xiu

AU - Hou, Ya Xin

AU - Cheng, Jia

AU - Guo, Jian Hua

AU - Wang, Shaun

AU - Khan, Ajab

AU - Sun, Na

AU - Li, Hongquan

N1 - Includes bibliographical references

PY - 2020/5

Y1 - 2020/5

N2 - Zearalenone (ZEA), a toxic substance produced by Fusarium fungi, accumulated in cereals grain and animal feed, causes injury to humans and animals. ZEA can induce obvious reproductive toxicity with the ovarian granulosa cells (GCs) as the main target. However, the study on exploring the protective compounds against ZEA-induced mouse primary ovarian GCs damage remains less. In the current study, the protective effect of 20 compounds derived from traditional Chinese medicines (TCMs) on the injury of mouse GCs caused by ZEA were evaluated using MTT assay and the cell morphology. Our results showed that chlorogenic acid (250, 500, and 1000 μg/mL) significantly suppress ZEA-induced GCs death. Western blot analysis suggested chlorogenic acid could rescue the up-regulated apoptosis of GCs induced by ZEA via attenuating the protein expression of cleaved caspase-3, the ratio of Bax/Bcl-2 and cleaved-PARP. Our results provide strong evidence that chlorogenic acid warrants further optimization for more potent and safer compounds for against the ZEA lead toxicity to humans and animals.

AB - Zearalenone (ZEA), a toxic substance produced by Fusarium fungi, accumulated in cereals grain and animal feed, causes injury to humans and animals. ZEA can induce obvious reproductive toxicity with the ovarian granulosa cells (GCs) as the main target. However, the study on exploring the protective compounds against ZEA-induced mouse primary ovarian GCs damage remains less. In the current study, the protective effect of 20 compounds derived from traditional Chinese medicines (TCMs) on the injury of mouse GCs caused by ZEA were evaluated using MTT assay and the cell morphology. Our results showed that chlorogenic acid (250, 500, and 1000 μg/mL) significantly suppress ZEA-induced GCs death. Western blot analysis suggested chlorogenic acid could rescue the up-regulated apoptosis of GCs induced by ZEA via attenuating the protein expression of cleaved caspase-3, the ratio of Bax/Bcl-2 and cleaved-PARP. Our results provide strong evidence that chlorogenic acid warrants further optimization for more potent and safer compounds for against the ZEA lead toxicity to humans and animals.

KW - Apoptosis

KW - Chlorogenic acid

KW - Injury

KW - Ovarian granulosa cells

KW - Zearalenone

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Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells

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