Chloroplast genome of serrated tussock (Nassella trichotoma): Structure and evolution

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    The genus Nassella contains many economically and environmentally important weed species in Australia, such as Nassella trichotoma (Nees) Hack. ex Arechav. (serrated tussock) and Nassella neesiana (Trin. & Rupr.) Barkworth (Chilean needle grass), which are both Weeds of National Significance (WoNS). Availability of Nassella chloroplast genome sequences can provide a versatile tool for identifying novel gene targets for DNA barcoding of these weeds species. We report here the first chloroplast genome of Nassella (N. trichotoma) obtained through de novo assembly of Illumina paired-end reads produced by total DNA sequencing. The N. trichotoma chloroplast genome is 112,102 bp in size, encodes 140 genes including 99 protein-coding genes, 37 tRNA genes and 4 ribosofmal RNA genes. The total size of intergenic regions within the genome is up to 48,764 bp. The total GC content of the genome is 37.88%, which is relatively lower than that of the reported genomes within the Stipeae tribe. A comparison of this chloro- plast genome with other Stipeae chloroplast genomes (Oryzopsis asperifolia Michx., Piptochaetium avenaceum (L.) Parodi, Achnatherum hymenoides Roem. & Schult., Stipa lipskyi Roshev. and Stipa purpurea Griseb.) provides new insights into their chloroplast genome evolution. Our future work will report genomes of other Nassella species as a means to identify novel DNA barcode regions useful for distinguishing these species.
    Original languageEnglish
    Title of host publicationProceedings of the 20th Australasian weeds conference (2016)
    Subtitle of host publicationScience, community and food Security: the weed challenge
    EditorsRod Randall, Sandy Lloyd, Catherine Borger
    Place of PublicationPerth
    PublisherWeed Society of Western Australia
    Number of pages5
    ISBN (Electronic)9780646960319
    Publication statusPublished - 2016
    Event20th Australasian Weeds Conference (2016) - Pan Pacific Hotel, Perth, Australia
    Duration: 11 Sept 201615 Sept 2016 (Conference website) (Conference proceedings)


    Conference20th Australasian Weeds Conference (2016)
    Abbreviated titleScience, Community and Food Security: the Weed Challenge
    OtherIn 2016, the Council of Australasian Weed Societies (CAWS), along with local hosts the Weeds Society of Western Australia, are celebrating the 20th Australasian Weeds Conference. The biennial conference is being held at the Pan Pacific in Perth, Western Australia which has traditionally been one of the highest attended AWC's, and over 300 delegates are expected from across Australasia.
    Delegates will come together to meet-up with peers, engage with industry sponsors, and to listen and participate in presentations and field trips on a variety of topics including:

    Herbicide Resistance
    Weeds of National Significance
    Agricultural weeds
    Environmental weeds
    Biological control of weeds
    Modelling and new technologies to manage weeds
    Legislation, regulation and policy to manage weed
    Internet address


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