TY - JOUR
T1 - Coexpression of p165 myeloid surface antigen and terminal deoxynucleotidyl transferase
T2 - a comparison of acute myeloid leukaemia and normal bone marrow cells
AU - Bradstock, K F
AU - Kerr, A
AU - Kabral, A
AU - Favaloro, E J
AU - Hewson, J W
PY - 1986/9
Y1 - 1986/9
N2 - A double immunofluorescence technique, using antibodies to terminal transferase (TdT) and a 165-kilodalton myeloid differentiation antigen (p165), has been used to investigate the phenomenon of TdT expression in cases of acute myeloid leukaemia (AML). Five cases of AML were shown to have significant (18-90%) numbers of leukaemic cells that concurrently expressed both TdT and p165 myeloid surface antigen. Examination of nonleukaemic bone marrow cells showed that the vast majority of normal TdT+ cells are p165 negative. However, in 5 of the 11 samples analyzed, rare cells staining for both p165 and TdT were found. These results suggest that some cases of TdT+ AML may arise from the clonal expansion of rare "biphenotypic" precursor cells existing in normal bone marrow.
AB - A double immunofluorescence technique, using antibodies to terminal transferase (TdT) and a 165-kilodalton myeloid differentiation antigen (p165), has been used to investigate the phenomenon of TdT expression in cases of acute myeloid leukaemia (AML). Five cases of AML were shown to have significant (18-90%) numbers of leukaemic cells that concurrently expressed both TdT and p165 myeloid surface antigen. Examination of nonleukaemic bone marrow cells showed that the vast majority of normal TdT+ cells are p165 negative. However, in 5 of the 11 samples analyzed, rare cells staining for both p165 and TdT were found. These results suggest that some cases of TdT+ AML may arise from the clonal expansion of rare "biphenotypic" precursor cells existing in normal bone marrow.
KW - Adult
KW - Aged
KW - Antigens, Neoplasm/analysis
KW - Antigens, Surface/analysis
KW - Bone Marrow/analysis
KW - Cell Differentiation
KW - DNA Nucleotidylexotransferase/analysis
KW - DNA Nucleotidyltransferases/analysis
KW - Female
KW - Fluorescent Antibody Technique
KW - Humans
KW - Leukemia, Myeloid, Acute/enzymology
KW - Male
KW - Middle Aged
KW - Neoplasm Proteins/analysis
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=woscharlessturt_pure&SrcAuth=WosAPI&KeyUT=WOS:A1986D512300006&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1002/ajh.2830230107
DO - 10.1002/ajh.2830230107
M3 - Article
C2 - 3526873
SN - 0361-8609
VL - 23
SP - 43
EP - 50
JO - American Journal of Hematology
JF - American Journal of Hematology
IS - 1
ER -
