Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 64-67 |
| Number of pages | 4 |
| Journal | Indian Journal of Veterinary Pathology |
| Volume | 37 |
| Issue number | 1 |
| Publication status | Published - 2013 |
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Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues. / Okuni, Julius Boniface; Lonzy, Ojok; Tsaousi, Panagiota; Loukopoulos, Panayiotis.
In: Indian Journal of Veterinary Pathology, Vol. 37, No. 1, 2013, p. 64-67.Research output: Contribution to journal › Article
TY - JOUR
T1 - Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues
AU - Okuni, Julius Boniface
AU - Lonzy, Ojok
AU - Tsaousi, Panagiota
AU - Loukopoulos, Panayiotis
N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Indian Journal of Veterinary Pathology. ISSNs: 0973-970X;
PY - 2013
Y1 - 2013
N2 - Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.
AB - Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.
KW - Bovine, immunohistochemistry, M. avium paratuberculosis, M. tuberculosis.
KW - veterinary diagnostic pathology
M3 - Article
VL - 37
SP - 64
EP - 67
JO - Indian Journal of Veterinary Pathology
JF - Indian Journal of Veterinary Pathology
SN - 0973-970X
IS - 1
ER -