Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues

Julius Boniface Okuni, Ojok Lonzy, Panagiota Tsaousi, Panayiotis Loukopoulos

Research output: Contribution to journalArticle

Abstract

Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.
Original languageEnglish
Pages (from-to)64-67
Number of pages4
JournalIndian Journal of Veterinary Pathology
Volume37
Issue number1
Publication statusPublished - 2013

Fingerprint

bovine tuberculosis
paratuberculosis
polyclonal antibodies
tuberculosis
mycobacterial diseases
antibodies
animal tissues
Mycobacterium
lesions (animal)
staining
tissues
screening
acids
cattle
bacteria
infection

Cite this

@article{5f21db23dcef4e43a7bfea69da53681c,
title = "Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues",
abstract = "Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.",
keywords = "Bovine, immunohistochemistry, M. avium paratuberculosis, M. tuberculosis., veterinary diagnostic pathology",
author = "Okuni, {Julius Boniface} and Ojok Lonzy and Panagiota Tsaousi and Panayiotis Loukopoulos",
note = "Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Indian Journal of Veterinary Pathology. ISSNs: 0973-970X;",
year = "2013",
language = "English",
volume = "37",
pages = "64--67",
journal = "Indian Journal of Veterinary Pathology",
issn = "0973-970X",
number = "1",

}

Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues. / Okuni, Julius Boniface; Lonzy, Ojok; Tsaousi, Panagiota; Loukopoulos, Panayiotis.

In: Indian Journal of Veterinary Pathology, Vol. 37, No. 1, 2013, p. 64-67.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues

AU - Okuni, Julius Boniface

AU - Lonzy, Ojok

AU - Tsaousi, Panagiota

AU - Loukopoulos, Panayiotis

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Indian Journal of Veterinary Pathology. ISSNs: 0973-970X;

PY - 2013

Y1 - 2013

N2 - Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.

AB - Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.

KW - Bovine, immunohistochemistry, M. avium paratuberculosis, M. tuberculosis.

KW - veterinary diagnostic pathology

M3 - Article

VL - 37

SP - 64

EP - 67

JO - Indian Journal of Veterinary Pathology

JF - Indian Journal of Veterinary Pathology

SN - 0973-970X

IS - 1

ER -