Comparative genome and transcriptome study of the gene expression difference between pathogenic and environmental strains of Prototheca zopfii

Xuanhao Zeng, Timothy Kudinha, Fanrong Kong, Qiang Qiang Zhang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Prototheca zopfii commonly exists in the environment, and causes invasive infections (protothecosis) in humans. The morbidity of protothecosis has increased rapidly in recent years, especially in systemic infections of patients with an impaired immune system. The infection in immunocompromised patients has a poor prognosis due to limited understanding of the pathogenesis of the disease, as most previous studies mainly focused on classification and recognition of pathogenic strains. In this study, we constructed the genome and transcriptome of two pathogenic strains and one environmental strain, by next generation sequencing methods. Based on our preliminary gene expression findings, genes in P. zopfii pathogenic strains are significantly up-regulated in metabolism in peroxisome, such as glyoxylate cycle, which may improve the organism’s resistance to the harsh environment in phagolysosome of macrophage and its ability to survive in an anaerobic environment. We also found some significant up-regulated genes, which are related to adherence and penetration in dermatophytes, and we speculate that this may enhance the virulence capacity of pathogenic strains. Finally, the genomes and transcriptomes of P. zopfii described here provide some base for further studies on the pathogenesis of this organism.

Original languageEnglish
Article number443
Pages (from-to)1-14
Number of pages14
JournalFrontiers in Microbiology
Volume10
Issue numberMAR
DOIs
Publication statusPublished - 07 Mar 2019

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Prototheca
Transcriptome
Genome
Gene Expression
Infection
Phagosomes
Arthrodermataceae
Peroxisomes
Immunocompromised Host
Genes
Virulence
Immune System
Macrophages
Morbidity

Cite this

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abstract = "Prototheca zopfii commonly exists in the environment, and causes invasive infections (protothecosis) in humans. The morbidity of protothecosis has increased rapidly in recent years, especially in systemic infections of patients with an impaired immune system. The infection in immunocompromised patients has a poor prognosis due to limited understanding of the pathogenesis of the disease, as most previous studies mainly focused on classification and recognition of pathogenic strains. In this study, we constructed the genome and transcriptome of two pathogenic strains and one environmental strain, by next generation sequencing methods. Based on our preliminary gene expression findings, genes in P. zopfii pathogenic strains are significantly up-regulated in metabolism in peroxisome, such as glyoxylate cycle, which may improve the organism’s resistance to the harsh environment in phagolysosome of macrophage and its ability to survive in an anaerobic environment. We also found some significant up-regulated genes, which are related to adherence and penetration in dermatophytes, and we speculate that this may enhance the virulence capacity of pathogenic strains. Finally, the genomes and transcriptomes of P. zopfii described here provide some base for further studies on the pathogenesis of this organism.",
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Comparative genome and transcriptome study of the gene expression difference between pathogenic and environmental strains of Prototheca zopfii. / Zeng, Xuanhao; Kudinha, Timothy; Kong, Fanrong; Zhang, Qiang Qiang.

In: Frontiers in Microbiology, Vol. 10, No. MAR, 443, 07.03.2019, p. 1-14.

Research output: Contribution to journalArticle

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