Complete nucleotide sequence of a native plasmid from Brevibacterium linens

Mathew Moore, Charles Svenson, David Bowling, Dianne Glenn

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Brevibacterium linens has commercial significance in the dairy industry and potential application in the production of bacteriocins and carotenoids. Strain development of these industrially significant organisms would be facilitated by the use of vectors, yet few are available. In this study we report the isolation of four novel plasmids from the Gram-positive coryneform B. linens, and determine the first complete nucleotide sequence of a native plasmid of B. linens. The cryptic plasmid pLIM is 7610bp in length, and belongs to a subfamily of ' replicating ColE2-related plasmids. Initial investigation suggests that replication in pLIM requires two replicases, a primase (RepA) and a DNA binding protein (RepB), encoded by a single operon repAB. The origin of replication is located upstream of repAB transcription.
Original languageEnglish
Pages (from-to)160-168
Number of pages9
JournalPlasmid
Volume49
Issue number2
DOIs
Publication statusPublished - 2003

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