Computational reclassification of resistance gene analogs in Rosaceae based on the NBS-LRR domain.

Angela M. Baldo, Suren Samuelian, Courtney A. Weber

    Research output: Book chapter/Published conference paperConference paperpeer-review

    Abstract

    Plant R genes are known to confer resistance to a variety of pathogens in a gene-for-gene mode. One hundred and ninety-five Rosaceae putative resistance gene analog (RGA) sequences containing conserved NBS-LRR domains were downloaded from the public domain. An additional seventy-six RGAs were amplified and sequenced from red raspberry Rubus idaeus cv 'Latham' using degenerate primers. Sequences were aligned using Hidden Markov Models and clustered with Neighbor-Joining. There are eleven TIR and ten non-TIR sequence clusters containing two or more genera with a bootstrap greater than 50%. This classification will be used to help identify novel RGAs found in the USDA-ARS apple and cherry germplasm collections in Geneva, NY.
    Original languageEnglish
    Title of host publicationPlant and Animal Genome XV Conference
    Place of PublicationUSA
    PublisherPlant and Animal Genome?
    Pages157 (P224)
    Publication statusPublished - 2007
    EventPlant and Animal Genome XV Conference - San Diego, USA, New Zealand
    Duration: 13 Jan 200717 Jan 2007

    Conference

    ConferencePlant and Animal Genome XV Conference
    Country/TerritoryNew Zealand
    Period13/01/0717/01/07

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