Computational reclassification of resistance gene analogs in Rosaceae based on the NBS-LRR domain.

Angela M. Baldo, Suren Samuelian, Courtney A. Weber

Research output: Book chapter/Published conference paperConference paperpeer-review

Abstract

Plant R genes are known to confer resistance to a variety of pathogens in a gene-for-gene mode. One hundred and ninety-five Rosaceae putative resistance gene analog (RGA) sequences containing conserved NBS-LRR domains were downloaded from the public domain. An additional seventy-six RGAs were amplified and sequenced from red raspberry Rubus idaeus cv 'Latham' using degenerate primers. Sequences were aligned using Hidden Markov Models and clustered with Neighbor-Joining. There are eleven TIR and ten non-TIR sequence clusters containing two or more genera with a bootstrap greater than 50%. This classification will be used to help identify novel RGAs found in the USDA-ARS apple and cherry germplasm collections in Geneva, NY.
Original languageEnglish
Title of host publicationPlant and Animal Genome XV Conference
Place of PublicationUSA
PublisherPlant and Animal Genome?
Pages157 (P224)
Publication statusPublished - 2007
EventPlant and Animal Genome XV Conference - San Diego, USA, New Zealand
Duration: 13 Jan 200717 Jan 2007

Conference

ConferencePlant and Animal Genome XV Conference
CountryNew Zealand
Period13/01/0717/01/07

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