Concurrent resistance and aerobic exercise stimulates both myofibrillar and mitochondrial protein synthesis in sedentary middle-aged men

Cheyne Donges, N.A. Burd, Rob Duffield, G.C. Smith, D.W.D West, M.J. Short, R. Mackenzie, L.D. Plank, P.R. Shephard, S.M. Phillips, J.A. Edge

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Abstract

We determined myofibrillar and mitochondrial protein fractional synthesis rates (FSR), intramuscular signaling protein phosphorylation, and mRNA expression responses after isolated bouts of resistance exercise (RE), aerobic exercise (AE), or in combination [termed concurrent exercise (CE)] in sedentary middle-aged men. Eight subjects (age = 53.3 ± 1.8 yr; body mass index = 29.4 ± 1.4 kg·m2) randomly completed 8 × 8 leg extension repetitions at 70% of one repetition-maximum, 40 min of cycling at 55% peak aerobic power output (AE), or (consecutively) 50% of the RE and AE trials (CE). Biopsies were obtained (during a primed, constant infusion of l-[ring-13C6]phenylalanine) while fasted, and at 1 and 4 h following postexercise ingestion of 20 g of protein. All trials increased mitochondrial FSR above fasted rates (RE = 1.3-fold; AE = 1.5; CE = 1.4; P < 0.05), although only CE (2.2) and RE (1.8) increased myofibrillar FSR (P < 0.05). At 1 h postexercise, phosphorylation of Akt on Ser473 (CE = 7.7; RE = 4.6) and Thr308 (CE = 4.4; RE = 2.9), and PRAS40 on Thr246 (CE = 3.8; AE = 2.5) increased (P < 0.05), with CE greater than AE for Akt Ser473-Thr308 and greater than RE for PRAS40 (P < 0.05). Despite increased phosphorylation of Akt-PRAS40, phosphorylation of mammalian target of rapamycin (Ser2448) remained unchanged (P > 0.05), while rpS6 (Ser235/236) increased only in RE (10.4) (P < 0.05). CE and AE both resulted in increased peroxisome proliferator receptor-' coactivator 1-' (PGC1') expression at 1 h (CE = 2.9; AE = 2.8; P < 0.05) and 4 h (CE = 2.6; AE = 2.4) and PGC1ß expression at 4 h (CE = 2.1; AE = 2.6; P < 0.05). These data suggest that CE-induced acute stimulation of myofibrillar and mitochondrial FSR, protein signaling, and mRNA expression are equivalent to either isolate mode (RE or AE). These results occurred without an interference effect on muscle protein subfractional synthesis rates, protein signaling, or mRNA expression.
Original languageEnglish
Pages (from-to)1992-2001
Number of pages10
JournalJournal of Applied Physiology
Volume112
Issue number12
DOIs
Publication statusPublished - Jun 2012

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Mitochondrial Proteins
Exercise
Messenger RNA
Proteins
Peroxisome Proliferators

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Donges, Cheyne ; Burd, N.A. ; Duffield, Rob ; Smith, G.C. ; West, D.W.D ; Short, M.J. ; Mackenzie, R. ; Plank, L.D. ; Shephard, P.R. ; Phillips, S.M. ; Edge, J.A. / Concurrent resistance and aerobic exercise stimulates both myofibrillar and mitochondrial protein synthesis in sedentary middle-aged men. In: Journal of Applied Physiology. 2012 ; Vol. 112, No. 12. pp. 1992-2001.
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abstract = "We determined myofibrillar and mitochondrial protein fractional synthesis rates (FSR), intramuscular signaling protein phosphorylation, and mRNA expression responses after isolated bouts of resistance exercise (RE), aerobic exercise (AE), or in combination [termed concurrent exercise (CE)] in sedentary middle-aged men. Eight subjects (age = 53.3 ± 1.8 yr; body mass index = 29.4 ± 1.4 kg{\^A}·m2) randomly completed 8 {\~A}— 8 leg extension repetitions at 70{\%} of one repetition-maximum, 40 min of cycling at 55{\%} peak aerobic power output (AE), or (consecutively) 50{\%} of the RE and AE trials (CE). Biopsies were obtained (during a primed, constant infusion of l-[ring-13C6]phenylalanine) while fasted, and at 1 and 4 h following postexercise ingestion of 20 g of protein. All trials increased mitochondrial FSR above fasted rates (RE = 1.3-fold; AE = 1.5; CE = 1.4; P < 0.05), although only CE (2.2) and RE (1.8) increased myofibrillar FSR (P < 0.05). At 1 h postexercise, phosphorylation of Akt on Ser473 (CE = 7.7; RE = 4.6) and Thr308 (CE = 4.4; RE = 2.9), and PRAS40 on Thr246 (CE = 3.8; AE = 2.5) increased (P < 0.05), with CE greater than AE for Akt Ser473-Thr308 and greater than RE for PRAS40 (P < 0.05). Despite increased phosphorylation of Akt-PRAS40, phosphorylation of mammalian target of rapamycin (Ser2448) remained unchanged (P > 0.05), while rpS6 (Ser235/236) increased only in RE (10.4) (P < 0.05). CE and AE both resulted in increased peroxisome proliferator receptor-' coactivator 1-' (PGC1') expression at 1 h (CE = 2.9; AE = 2.8; P < 0.05) and 4 h (CE = 2.6; AE = 2.4) and PGC1{\~A}Ÿ expression at 4 h (CE = 2.1; AE = 2.6; P < 0.05). These data suggest that CE-induced acute stimulation of myofibrillar and mitochondrial FSR, protein signaling, and mRNA expression are equivalent to either isolate mode (RE or AE). These results occurred without an interference effect on muscle protein subfractional synthesis rates, protein signaling, or mRNA expression.",
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Donges, C, Burd, NA, Duffield, R, Smith, GC, West, DWD, Short, MJ, Mackenzie, R, Plank, LD, Shephard, PR, Phillips, SM & Edge, JA 2012, 'Concurrent resistance and aerobic exercise stimulates both myofibrillar and mitochondrial protein synthesis in sedentary middle-aged men', Journal of Applied Physiology, vol. 112, no. 12, pp. 1992-2001. https://doi.org/10.1152/japplphysiol.00166.2012

Concurrent resistance and aerobic exercise stimulates both myofibrillar and mitochondrial protein synthesis in sedentary middle-aged men. / Donges, Cheyne; Burd, N.A.; Duffield, Rob; Smith, G.C.; West, D.W.D; Short, M.J.; Mackenzie, R.; Plank, L.D.; Shephard, P.R.; Phillips, S.M.; Edge, J.A.

In: Journal of Applied Physiology, Vol. 112, No. 12, 06.2012, p. 1992-2001.

Research output: Contribution to journalArticle

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T1 - Concurrent resistance and aerobic exercise stimulates both myofibrillar and mitochondrial protein synthesis in sedentary middle-aged men

AU - Donges, Cheyne

AU - Burd, N.A.

AU - Duffield, Rob

AU - Smith, G.C.

AU - West, D.W.D

AU - Short, M.J.

AU - Mackenzie, R.

AU - Plank, L.D.

AU - Shephard, P.R.

AU - Phillips, S.M.

AU - Edge, J.A.

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N2 - We determined myofibrillar and mitochondrial protein fractional synthesis rates (FSR), intramuscular signaling protein phosphorylation, and mRNA expression responses after isolated bouts of resistance exercise (RE), aerobic exercise (AE), or in combination [termed concurrent exercise (CE)] in sedentary middle-aged men. Eight subjects (age = 53.3 ± 1.8 yr; body mass index = 29.4 ± 1.4 kg·m2) randomly completed 8 × 8 leg extension repetitions at 70% of one repetition-maximum, 40 min of cycling at 55% peak aerobic power output (AE), or (consecutively) 50% of the RE and AE trials (CE). Biopsies were obtained (during a primed, constant infusion of l-[ring-13C6]phenylalanine) while fasted, and at 1 and 4 h following postexercise ingestion of 20 g of protein. All trials increased mitochondrial FSR above fasted rates (RE = 1.3-fold; AE = 1.5; CE = 1.4; P < 0.05), although only CE (2.2) and RE (1.8) increased myofibrillar FSR (P < 0.05). At 1 h postexercise, phosphorylation of Akt on Ser473 (CE = 7.7; RE = 4.6) and Thr308 (CE = 4.4; RE = 2.9), and PRAS40 on Thr246 (CE = 3.8; AE = 2.5) increased (P < 0.05), with CE greater than AE for Akt Ser473-Thr308 and greater than RE for PRAS40 (P < 0.05). Despite increased phosphorylation of Akt-PRAS40, phosphorylation of mammalian target of rapamycin (Ser2448) remained unchanged (P > 0.05), while rpS6 (Ser235/236) increased only in RE (10.4) (P < 0.05). CE and AE both resulted in increased peroxisome proliferator receptor-' coactivator 1-' (PGC1') expression at 1 h (CE = 2.9; AE = 2.8; P < 0.05) and 4 h (CE = 2.6; AE = 2.4) and PGC1ß expression at 4 h (CE = 2.1; AE = 2.6; P < 0.05). These data suggest that CE-induced acute stimulation of myofibrillar and mitochondrial FSR, protein signaling, and mRNA expression are equivalent to either isolate mode (RE or AE). These results occurred without an interference effect on muscle protein subfractional synthesis rates, protein signaling, or mRNA expression.

AB - We determined myofibrillar and mitochondrial protein fractional synthesis rates (FSR), intramuscular signaling protein phosphorylation, and mRNA expression responses after isolated bouts of resistance exercise (RE), aerobic exercise (AE), or in combination [termed concurrent exercise (CE)] in sedentary middle-aged men. Eight subjects (age = 53.3 ± 1.8 yr; body mass index = 29.4 ± 1.4 kg·m2) randomly completed 8 × 8 leg extension repetitions at 70% of one repetition-maximum, 40 min of cycling at 55% peak aerobic power output (AE), or (consecutively) 50% of the RE and AE trials (CE). Biopsies were obtained (during a primed, constant infusion of l-[ring-13C6]phenylalanine) while fasted, and at 1 and 4 h following postexercise ingestion of 20 g of protein. All trials increased mitochondrial FSR above fasted rates (RE = 1.3-fold; AE = 1.5; CE = 1.4; P < 0.05), although only CE (2.2) and RE (1.8) increased myofibrillar FSR (P < 0.05). At 1 h postexercise, phosphorylation of Akt on Ser473 (CE = 7.7; RE = 4.6) and Thr308 (CE = 4.4; RE = 2.9), and PRAS40 on Thr246 (CE = 3.8; AE = 2.5) increased (P < 0.05), with CE greater than AE for Akt Ser473-Thr308 and greater than RE for PRAS40 (P < 0.05). Despite increased phosphorylation of Akt-PRAS40, phosphorylation of mammalian target of rapamycin (Ser2448) remained unchanged (P > 0.05), while rpS6 (Ser235/236) increased only in RE (10.4) (P < 0.05). CE and AE both resulted in increased peroxisome proliferator receptor-' coactivator 1-' (PGC1') expression at 1 h (CE = 2.9; AE = 2.8; P < 0.05) and 4 h (CE = 2.6; AE = 2.4) and PGC1ß expression at 4 h (CE = 2.1; AE = 2.6; P < 0.05). These data suggest that CE-induced acute stimulation of myofibrillar and mitochondrial FSR, protein signaling, and mRNA expression are equivalent to either isolate mode (RE or AE). These results occurred without an interference effect on muscle protein subfractional synthesis rates, protein signaling, or mRNA expression.

U2 - 10.1152/japplphysiol.00166.2012

DO - 10.1152/japplphysiol.00166.2012

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SP - 1992

EP - 2001

JO - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology

JF - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology

SN - 1522-1601

IS - 12

ER -