Quolls are carnivorous marsupials in the family Dasyuridae with characteristic white spots. They are distributed throughout Australia and New Guinea, but uncommonly seen due to their mostly nocturnal solitary nature, and large home ranges. All Australian quolls are listed as ‘near threatened’ or ‘endangered’ at state, national and international levels, largely due to human-induced threats. Threats include introduced predators, habitat loss through clearing and modifications including changed fire regimes, disease, human persecution, vehicle collisions and accidental or targeted poisoning by humans and cane toads (Rhinella marina). Conservation efforts that have focussed on reducing introduced predators, and minimising the impact of cane toads, have aided some translocations, hence species recovery in some local areas of Australia has occurred. Where species conservation has required captive breeding for translocation, successful captive management has been crucial. We summarise research conducted in captivity on aspects of birth and development, health and disease, and blood and nutrition parameters of quolls, and suggest future directions for research. Further research on captive and wild quoll populations will benefit future translocations, reintroductions and conservation through increased knowledge, improved maintenance and husbandry of captive colonies, and monitoring of wild populations.