Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

Cina Vipin; David Luckett; John Harper; Gavin Ash; Andrzej Kilian; Simon R. Ellwood; T. Huyen; Harsh Raman

doi:10.1270/jsbbs.63.292

Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

Cina Vipin, David Luckett, John Harper, Gavin Ash, Andrzej Kilian, Simon R. Ellwood, T. Huyen, Harsh Raman

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Abstract

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.

Original language	English
Pages (from-to)	292-300
Number of pages	9
Journal	Breeding Science
Volume	63
Issue number	3
DOIs	https://doi.org/10.1270/jsbbs.63.292
Publication status	Published - 2013

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title = "Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)",

abstract = "We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.",

keywords = "Open access version available, Diversity Array Technology, Genetic linkage mapping, Lupinus albus, Molecular markers",

author = "Cina Vipin and David Luckett and John Harper and Gavin Ash and Andrzej Kilian and Ellwood, {Simon R.} and T. Huyen and Harsh Raman",

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T1 - Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

AU - Vipin, Cina

AU - Luckett, David

AU - Harper, John

AU - Ash, Gavin

AU - Kilian, Andrzej

AU - Ellwood, Simon R.

AU - Huyen, T.

AU - Raman, Harsh

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Breeding Science. ISSNs: 1344-7610;

PY - 2013

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N2 - We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.

AB - We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.

KW - Open access version available

KW - Diversity Array Technology

KW - Genetic linkage mapping

KW - Lupinus albus

KW - Molecular markers

U2 - 10.1270/jsbbs.63.292

DO - 10.1270/jsbbs.63.292

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C2 - 24273424

SN - 1344-7610

VL - 63

SP - 292

EP - 300

JO - Breeding Science

JF - Breeding Science

IS - 3

ER -

Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

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