Crystallization of the flexible nuclear import receptor importin-ß in the unliganded state

Noelia Roman, Brenda Kirkby, M. Marfori, B. Kobe, Jade Forwood

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The transport of macromolecules across the nuclear envelope is an essential eukaryotic process that enables proteins such as transcription factors, polymerases and histones to gain access to the genetic material contained within the nucleus. Importin- plays a central role in the nucleocytoplasmic transport process, mediating nuclear import through a range of interactions with cytoplasmic, nuclear and nuclear pore proteins such as importin-, Ran, nucleoporins and various cargo molecules. The unliganded form of the full-length yeast importin- has been expressed and crystallized. The crystals were obtained by vapour diffusion at pH 6.5 and 290 K. The crystals belonged to space group P21 (unit-cell parameters a = 58.17, b = 127.25, c = 68.52 Ã…, = 102.23). One molecule is expected in the asymmetric unit. The crystals diffracted to 2.4 Ã… resolution using a laboratory X-ray source and were suitable for crystal structure determination.
Original languageEnglish
Pages (from-to)625-628
Number of pages4
JournalActa Crystallographica Section F:Structural Biology Communications
Volume65
Issue number6
DOIs
Publication statusPublished - 2009

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