TY - JOUR
T1 - Cucumber mosaic virus RNA 5 is a mixed population derived from the conserved 3′-terminal regions of genomic RNAs 2 and 3
AU - Blanchard, Christopher L.
AU - Boyce, Patricia M.
AU - Anderson, Beau J.
PY - 1996/3/15
Y1 - 1996/3/15
N2 - Analysis of encapsidated RNA from the Q strain of CMV (Q-CMV) has indicated the presence of a discreet population of molecules of approximately 300 nt termed RNA 5 (Peden, K. W. C., and Symons, R. H., Virology 53, 487-492, 1973.). Q-CMV RNA 5 was isolated and the 5′-end sequence was determined by direct RNA sequencing. This sequence corresponded to the exact beginning of the imperfectly conserved 3′-terminal region of genomic RNAs 1, 2, and 3. A probe generated from RNA 3 consisting of the last 130 nt of this region hybridised to RNA 5. Oligonucleotides containing sequences from the 5′-and 3′-ends of the conserved region were used to generate RNA 5 cDNA clones by RT-PCR on gel-purified RNA 5. Sequencing of these clones and primer extension analysis of transcripts (derived from the cDNA clones) and purified RNA 5 indicated that RNA 5 consists of the conserved 3′-terminal region of genomic RNAs 2 and 3.
AB - Analysis of encapsidated RNA from the Q strain of CMV (Q-CMV) has indicated the presence of a discreet population of molecules of approximately 300 nt termed RNA 5 (Peden, K. W. C., and Symons, R. H., Virology 53, 487-492, 1973.). Q-CMV RNA 5 was isolated and the 5′-end sequence was determined by direct RNA sequencing. This sequence corresponded to the exact beginning of the imperfectly conserved 3′-terminal region of genomic RNAs 1, 2, and 3. A probe generated from RNA 3 consisting of the last 130 nt of this region hybridised to RNA 5. Oligonucleotides containing sequences from the 5′-and 3′-ends of the conserved region were used to generate RNA 5 cDNA clones by RT-PCR on gel-purified RNA 5. Sequencing of these clones and primer extension analysis of transcripts (derived from the cDNA clones) and purified RNA 5 indicated that RNA 5 consists of the conserved 3′-terminal region of genomic RNAs 2 and 3.
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U2 - 10.1006/viro.1996.0155
DO - 10.1006/viro.1996.0155
M3 - Article
C2 - 8610452
AN - SCOPUS:0029947182
SN - 0042-6822
VL - 217
SP - 598
EP - 601
JO - Virology
JF - Virology
IS - 2
ER -