Abstract
In order to elucidate the mechanism of action ofcurcumin against hepatic fibrosis, cultured rat hepatic stellatecells (HSC) (HSC-T6) were incubated with curcumin for24 h, after which apoptosis was measured by flow-cytometry.The protein levels of the pro-apoptotic factors Fas andp53b as well as of the anti-apoptotic factor Bcl-2 were monitoredby immunocytochemical ABC staining after incubationwith curcumin for 24 h. In the case of 20 µM curcumin,not only was the respective apoptosis index increased, butalso the abundance of the pro-apoptotic factors Fas andp53 were amplified, whereas that of the anti-apoptoticfactor Bcl-2 decreased. All these effects were highly reproducible (P<0.05). Consequently, curcumin has an up-regulating effect on pro-apoptotic factors like Fas and p53 as well as a down-regulating effect of the anti-apoptotic factor Bcl-2, thus inducing apoptosis in HSC.
Original language | English |
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Pages (from-to) | 281-285 |
Number of pages | 5 |
Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
Volume | 70 |
Issue number | 11-12 |
DOIs | |
Publication status | Published - Nov 2015 |