In order to elucidate the mechanism of action ofcurcumin against hepatic fibrosis, cultured rat hepatic stellatecells (HSC) (HSC-T6) were incubated with curcumin for24 h, after which apoptosis was measured by flow-cytometry.The protein levels of the pro-apoptotic factors Fas andp53b as well as of the anti-apoptotic factor Bcl-2 were monitoredby immunocytochemical ABC staining after incubationwith curcumin for 24 h. In the case of 20 ÂµM curcumin,not only was the respective apoptosis index increased, butalso the abundance of the pro-apoptotic factors Fas andp53 were amplified, whereas that of the anti-apoptoticfactor Bcl-2 decreased. All these effects were highly reproducible(P?
|Number of pages||5|
|Journal||Zeitschrift fur Naturforschung - Section C Journal of Biosciences|
|Publication status||Published - Nov 2015|
He, Y-J., Kuchta, K., Lv, X., Lin, Y., Ye, G-R., Liu, X-Y., Song, H-D., Wang, L., Kobayashi, Y., & Shu, J-C. (2015). Curcumin: The main active constituent of turmeric (Curcuma longa L.) : induces apoptosis in hepatic stellate cells by modulating the abundance of apoptosis-related growth factors. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 70(11-12), 281-285. https://doi.org/10.1515/znc-2015-4143