TY - JOUR
T1 - Curcumol inhibits encephalomyocarditis virus by promoting IFN-β secretion
AU - Zheng, Jiangang
AU - Xu, Yinlan
AU - Khan, Ajab
AU - Sun, Panpan
AU - Sun, Yaogui
AU - Fan, Kuohai
AU - Yin, Wei
AU - Wang, Shaoyu
AU - Li, Hongquan
AU - Sun, Na
N1 - Funding Information:
This work was supported by the National Key R&D Program of China (grant number 2017YFD0501500), Shanxi Province Excellent Doctoral Work Award-Scientific Research Project (SXYBKY2019051) and Science and Technology Innovation Project of Colleges and Universities in Shanxi Province (2020L0139).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - BackgroundEncephalomyocarditis
virus (EMCV) infection can cause reproductive failure in sows and acute
myocarditis and sudden death in piglets. It has caused huge economic
losses to the global pig industry and that is why it is necessary to
develop effective new treatment compounds. Zedoary turmeric oil has been
used for treating myocarditis. Curcumol extracted from the roots of
curcuma is one of the main active ingredient of zedoary turmeric oil.
The anti-EMCV activity of curcumol along with the molecular mechanisms
involved with a focus on IFN-β signaling pathway was investigated in
this study.Method3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay was used to determine the maximum non-toxic
concentration (MNTC), 50% cytotoxic concentration (CC50), maximum inhibition rate (MIR) and 50% effective concentration (EC50)
against EMCV. Through EMCV load, the anti-viral effect of curcumol was
quantitatively determined using real-time quantitative PCR (qPCR). The
effect of curcumol on the expression of IFN-β was investigated using
real-time quantitative PCR and ELISA. Western blot was used to determine
the amounts of MDA5, MAVS, TANK, IRF3 and P-IRF3 proteins in human
embryonic kidney 293 T (HEK-293 T) cells infected with EMCV.ResultsThe
results of MTT showed that compared with the ribavirin positive control
group, the maximum inhibition ratio (MIR) of curcumol was greater but
the selection index (SI) value was much smaller than that of ribavirin.
The results of qPCR showed that curcumol and ribavirin significantly
reduced the replication of EMCV in HEK-293 T cells. The curcumol
(0.025 mg/mL) treatment has significantly increased IFN-β mRNA
expression in the EMCV-infected HEK-293 T cells while ribavirin
treatment did not. The results of ELISA showed that curcumol
(0.025 mg/mL and 0.0125 mg/mL) has significantly increased the
expression of IFN-β protein in EMCV-infected HEK-293 T cells. The
results of Western blot showed that curcumol can inhibit the degradation
of TANK protein mediated by EMCV and promote the expression of MDA5 and
P-IRF3, while the protein expression level of MAVS and IRF3 remain
unchanged.ConclusionCurcumol has biological activity against EMCV which we suggest that IFN-β signaling pathway is one of its mechanisms.
AB - BackgroundEncephalomyocarditis
virus (EMCV) infection can cause reproductive failure in sows and acute
myocarditis and sudden death in piglets. It has caused huge economic
losses to the global pig industry and that is why it is necessary to
develop effective new treatment compounds. Zedoary turmeric oil has been
used for treating myocarditis. Curcumol extracted from the roots of
curcuma is one of the main active ingredient of zedoary turmeric oil.
The anti-EMCV activity of curcumol along with the molecular mechanisms
involved with a focus on IFN-β signaling pathway was investigated in
this study.Method3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay was used to determine the maximum non-toxic
concentration (MNTC), 50% cytotoxic concentration (CC50), maximum inhibition rate (MIR) and 50% effective concentration (EC50)
against EMCV. Through EMCV load, the anti-viral effect of curcumol was
quantitatively determined using real-time quantitative PCR (qPCR). The
effect of curcumol on the expression of IFN-β was investigated using
real-time quantitative PCR and ELISA. Western blot was used to determine
the amounts of MDA5, MAVS, TANK, IRF3 and P-IRF3 proteins in human
embryonic kidney 293 T (HEK-293 T) cells infected with EMCV.ResultsThe
results of MTT showed that compared with the ribavirin positive control
group, the maximum inhibition ratio (MIR) of curcumol was greater but
the selection index (SI) value was much smaller than that of ribavirin.
The results of qPCR showed that curcumol and ribavirin significantly
reduced the replication of EMCV in HEK-293 T cells. The curcumol
(0.025 mg/mL) treatment has significantly increased IFN-β mRNA
expression in the EMCV-infected HEK-293 T cells while ribavirin
treatment did not. The results of ELISA showed that curcumol
(0.025 mg/mL and 0.0125 mg/mL) has significantly increased the
expression of IFN-β protein in EMCV-infected HEK-293 T cells. The
results of Western blot showed that curcumol can inhibit the degradation
of TANK protein mediated by EMCV and promote the expression of MDA5 and
P-IRF3, while the protein expression level of MAVS and IRF3 remain
unchanged.ConclusionCurcumol has biological activity against EMCV which we suggest that IFN-β signaling pathway is one of its mechanisms.
KW - Antivirus
KW - Curcumol
KW - EMCV
KW - IFN-β
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U2 - 10.1186/s12917-021-03015-4
DO - 10.1186/s12917-021-03015-4
M3 - Article
C2 - 34587973
AN - SCOPUS:85116080928
SN - 1746-6148
VL - 17
SP - 1
EP - 9
JO - BMC Veterinary Research
JF - BMC Veterinary Research
IS - 1
M1 - 318
ER -