Cyclosporine diminishes multidrug resistance in K562/ADM cells and improves complete remission in patients with acute myeloid leukemia

Guang-Yao Li, Ji-Zhu Liu, Bin Zhang, Lexin Wang, Chun-bo Wang, Shou-Guo Chen

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Abstract

This study was designed to investigate the effects of cyclosporine A (CsA) on a multidrug-resistance cultured cell line, and its effect on complete remission in patients with acute myeloid leukemia (AML). A multidrug resistant K562/ADM cell line and drug-sensitive K562 cell line was used. The intracellular concentration of daunorubicin and the accumulation of Rhodamine 123 (Rh123) in the K562/ADM and K562 cells were evaluated. Clinical effects of CsA were also studied in 65 patients with AML. In the K562/ADM cells, the 50% of inhibition concentration (IC50) of daunorubicin only group was 23.0±5.2'mol/L, which was greater than in other groups co-administered with CsA (1.2±4.8 'mol/L), verapamil (1.5±5.4'mol/L) or CsA+verapamil (1.4±4.3'mol/L) (all P<0.01). The relative fluorescence intensity of Rh123 in the K562/ADM cells treated with CsA and daunorubicin was increased from 48.9% to 69.8% (P<0.05). CsA also improved the complete remission rate in the AML patients (72.7% vs 21.9%, P<0.01). We conclude that CsA can significantly diminish the multidrug resistance in K562/ADM cells. It also enhances the complete remission rates in patients with AML. CsA may be used as an integral part of the chemotherapy for AML.
Original languageEnglish
Pages (from-to)566-570
Number of pages5
JournalBiomedicine and Pharmacotherapy
Volume63
Issue number8
DOIs
Publication statusPublished - 2009

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