Degradable chitosan-collagen composites seeded with cells as tissue engineered heart valves

Jian-Hua Fu, Man Zhao, Yan-Rong Lin, Xu-Dong Tian, Ya-Dong Wang, Zhen-Xing Wang, Lexin Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves. The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds. The adhesion and biological activities of the seeded cells were also investigated. Methods: Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells. After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material. After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material. Results: The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells. The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01). Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells. Conclusions: Endothelial cells can be differentiated from BMSCs when seeded onto the collagen-chitosan composite scaffolds. The seeded cells retained their biological activity after being cultured in vitro and seeded into the collagen-chitosan composite material.
Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalHeart Lung and Circulation
Volume26
Issue number1
Early online dateJul 2016
DOIs
Publication statusPublished - 2017

Fingerprint Dive into the research topics of 'Degradable chitosan-collagen composites seeded with cells as tissue engineered heart valves'. Together they form a unique fingerprint.

  • Cite this