Design of a chimeric DNA vaccine against Brucella spp.

M. Abkar, A.S. Lofti, J. Amani, Seyed Ghorashi, G.N. Brujeni, M. Kamali

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Brucellosis, a common illness between domestic animals and humans, is mostly caused by Brucella (B) abortus, B. melitensis and B.suis. It has been shown vaccination of mice by Omp19 and Omp31 can induce protection against different Brucella spp. Furthermore, urease enzyme is considered a virulence factor in most members of Brucella. Here, we designed a chimeric DNA vaccine containing omp19, omp31 and urease genes to obtain high protection against Brucella spp. The construct was analyzed using bioinformatics tools. Linear and discontinuous B-cell epitopes, MHC class I and II binding peptides of the chimeric vaccine were predicted. Results suggest the construct can be an appropriate vaccine candidate against brucellosis.
Original languageEnglish
Pages (from-to)223-233
Number of pages11
JournalMinerva Biotecnologica
Volume26
Issue number4
Publication statusPublished - Dec 2014

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