TY - GEN
T1 - Detecting exosomes specifically
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
AU - Shiddiky, Muhammad J.A.
AU - Vaidyanathan, Ramanathan
AU - Naghibosadat, Maedeh
AU - Rauf, Sakandar
AU - Korbie, Darren
AU - Carrascosa, Laura G.
AU - Trau, Matt
N1 - Publisher Copyright:
© 14CBMS.
PY - 2014
Y1 - 2014
N2 - We report a multiplexed microfluidic device for highly specific capture and detection of multiple exosome targets using a tuneable alternating current electrohydrodynamics (ac-EHD) forces - referred to as nanoshearing [1,2]. These forces generated within few nanometers of an electrode surface (i.e., double layer) to generate nanoscaled fluid flow that enhances the specificity of capture and also remove the nonspecifically adsorbed species from the electrode surface. To demonstrate the utility of this phenomenon, we present data on purpose-built microfluidic devices that employ ac-EHD induced surface shear forces to specifically capture exosomes isolated from complex biological fluids (e.g., cell culture media, serum etc.) [3].
AB - We report a multiplexed microfluidic device for highly specific capture and detection of multiple exosome targets using a tuneable alternating current electrohydrodynamics (ac-EHD) forces - referred to as nanoshearing [1,2]. These forces generated within few nanometers of an electrode surface (i.e., double layer) to generate nanoscaled fluid flow that enhances the specificity of capture and also remove the nonspecifically adsorbed species from the electrode surface. To demonstrate the utility of this phenomenon, we present data on purpose-built microfluidic devices that employ ac-EHD induced surface shear forces to specifically capture exosomes isolated from complex biological fluids (e.g., cell culture media, serum etc.) [3].
KW - Electrohydrodynamics
KW - Exosomes
KW - Nonspecific adsorption
KW - Shear force
UR - http://www.scopus.com/inward/record.url?scp=84941662975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941662975&partnerID=8YFLogxK
M3 - Conference paper
AN - SCOPUS:84941662975
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 674
EP - 676
BT - Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
PB - Chemical and Biological Microsystems Society
Y2 - 26 October 2014 through 30 October 2014
ER -