Detection and genotyping of Campylobacter species in broilers meat

Campylobacter is a gram-negative bacteria with at least 16 different species which some of these species are important in food-borne disease in human. Campylobacter is the most important cause of human bacterial gastroenteritis particularly in developed countries. Campylobacter coli (C. coli) and C. jejuni are the two most common species that are reported from enteric infections in Australia and usually are associated with poultry meat as contamination of carcasses may occur at processing plants. In this study, a rapid molecular diagnostic and genotyping method was developed to detect and differentiate C. coli and C. jejuni in broilers carcasses. The test was evaluated further by investigating additional human samples. The sensitivity and specificity of the test was 100% and 92%, respectively.