Detection of regional DNA methylation using DNA-graphene affinity interactions

Md Hakimul Haque, Vinod Gopalan, Sharda Yadav, Md Nazmul Islam, Ehsan Eftekhari, Qin Li, Laura G. Carrascosa, Nam Trung Nguyen, Alfred K. Lam, Muhammad J.A. Shiddiky

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
Original languageEnglish
Pages (from-to)615-621
Number of pages7
JournalBiosensors and Bioelectronics
Early online dateSept 2016
Publication statusPublished - 15 Jan 2017


Dive into the research topics of 'Detection of regional DNA methylation using DNA-graphene affinity interactions'. Together they form a unique fingerprint.

Cite this