TY - JOUR
T1 - Detection of regional DNA methylation using DNA-graphene affinity interactions
AU - Haque, Md Hakimul
AU - Gopalan, Vinod
AU - Yadav, Sharda
AU - Islam, Md Nazmul
AU - Eftekhari, Ehsan
AU - Li, Qin
AU - Carrascosa, Laura G.
AU - Nguyen, Nam Trung
AU - Lam, Alfred K.
AU - Shiddiky, Muhammad J.A.
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/1/15
Y1 - 2017/1/15
N2 - We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
AB - We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
KW - Disposable screen-printed electrode
KW - DNA methylation
KW - Electrochemical detection
KW - Graphene-DNA base interaction
KW - Regional methylation
UR - http://www.scopus.com/inward/record.url?scp=84986301163&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986301163&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.09.016
DO - 10.1016/j.bios.2016.09.016
M3 - Article
C2 - 27616287
AN - SCOPUS:84986301163
SN - 0956-5663
VL - 87
SP - 615
EP - 621
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -
