TY - JOUR
T1 - Detection of Single Nucleotide Variations in Viral RNA Populations by Primer Extension
AU - Blanchard, Christopher L.
AU - Anderson, Beau J.
PY - 1998
Y1 - 1998
N2 - A method for identifying and differentiating between two highly similar sequence variants in the cucumber mosaic virus RNA 5 population is described. The technique is based on the use of different primers with 3′ terminal mismatches for primer extension analysis. Primers varied in their length, number of 3′ mismatches, and sequence context of the mismatch. The results indicate that the size of the primer is critically important to the ability of this method to differentiate between highly similar RNAs. This technique should prove very useful for the genetic analysis of characteristics that are determined by single nucleotide variations in viral RNA genomes.
AB - A method for identifying and differentiating between two highly similar sequence variants in the cucumber mosaic virus RNA 5 population is described. The technique is based on the use of different primers with 3′ terminal mismatches for primer extension analysis. Primers varied in their length, number of 3′ mismatches, and sequence context of the mismatch. The results indicate that the size of the primer is critically important to the ability of this method to differentiate between highly similar RNAs. This technique should prove very useful for the genetic analysis of characteristics that are determined by single nucleotide variations in viral RNA genomes.
KW - CMV
KW - Primer extension
KW - RNA virus
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U2 - 10.1023/A:1007464523687
DO - 10.1023/A:1007464523687
M3 - Article
AN - SCOPUS:0032375798
SN - 0735-9640
VL - 16
SP - 33
EP - 40
JO - Plant Molecular Biology Reporter
JF - Plant Molecular Biology Reporter
IS - 1
ER -