Determination of sucrose in equine serum using liquid chromatography-mass spectrometry (LC/MS)

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Abstract

Mucosal integrity may be objectively assessed by determination of the absorption of exogenous substances such as sucrose. Gas chromatographyï'½mass spectrometry (GC/MS) and liquid chromatographyï'½mass spectrometry (LC/MS) have been reported for the accurate quantification of low concentrations of sucrose in serum. LC/MS offered the advantage of high sensitivity and mass selectivity without the need for extensive sample derivatization required for GC/MS methods. However, the high polarity and non-volatile nature of the sucrose molecule renders LC/MS techniques challenging. Previously published reports lacked sufficient detail to permit replication of methodology. Problems encountered with existing protocols included poor peak resolution and weak fragmentation of the parent molecule. This communication describes a LC/MS protocol developed to provide improved resolution and product detection.
Original languageEnglish
Pages (from-to)3668-3671
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume879
Issue number30
DOIs
Publication statusPublished - Nov 2011

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Liquid chromatography
Liquid Chromatography
Horses
Mass spectrometry
Sucrose
Mass Spectrometry
Serum
Gas Chromatography-Mass Spectrometry
Gas chromatography
Molecules
Communication

Cite this

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title = "Determination of sucrose in equine serum using liquid chromatography-mass spectrometry (LC/MS)",
abstract = "Mucosal integrity may be objectively assessed by determination of the absorption of exogenous substances such as sucrose. Gas chromatography{\~A}¯'{\^A}½mass spectrometry (GC/MS) and liquid chromatography{\~A}¯'{\^A}½mass spectrometry (LC/MS) have been reported for the accurate quantification of low concentrations of sucrose in serum. LC/MS offered the advantage of high sensitivity and mass selectivity without the need for extensive sample derivatization required for GC/MS methods. However, the high polarity and non-volatile nature of the sucrose molecule renders LC/MS techniques challenging. Previously published reports lacked sufficient detail to permit replication of methodology. Problems encountered with existing protocols included poor peak resolution and weak fragmentation of the parent molecule. This communication describes a LC/MS protocol developed to provide improved resolution and product detection.",
keywords = "Open access version available, Liquid chromatography'mass spectrometry, Peak resolution, Sodiated sucrose",
author = "Eliza D'Arcy-Moskwa and Weston, {Leslie A.} and Glenys Noble and Sharanne Raidal",
note = "Imported on 12 Apr 2017 - DigiTool details were: month (773h) = November, 2011; Journal title (773t) = Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. ISSNs: 1570-0232;",
year = "2011",
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doi = "10.1016/j.jchromb.2011.09.049",
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issn = "1570-0232",
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T1 - Determination of sucrose in equine serum using liquid chromatography-mass spectrometry (LC/MS)

AU - D'Arcy-Moskwa, Eliza

AU - Weston, Leslie A.

AU - Noble, Glenys

AU - Raidal, Sharanne

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = November, 2011; Journal title (773t) = Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. ISSNs: 1570-0232;

PY - 2011/11

Y1 - 2011/11

N2 - Mucosal integrity may be objectively assessed by determination of the absorption of exogenous substances such as sucrose. Gas chromatographyï'½mass spectrometry (GC/MS) and liquid chromatographyï'½mass spectrometry (LC/MS) have been reported for the accurate quantification of low concentrations of sucrose in serum. LC/MS offered the advantage of high sensitivity and mass selectivity without the need for extensive sample derivatization required for GC/MS methods. However, the high polarity and non-volatile nature of the sucrose molecule renders LC/MS techniques challenging. Previously published reports lacked sufficient detail to permit replication of methodology. Problems encountered with existing protocols included poor peak resolution and weak fragmentation of the parent molecule. This communication describes a LC/MS protocol developed to provide improved resolution and product detection.

AB - Mucosal integrity may be objectively assessed by determination of the absorption of exogenous substances such as sucrose. Gas chromatographyï'½mass spectrometry (GC/MS) and liquid chromatographyï'½mass spectrometry (LC/MS) have been reported for the accurate quantification of low concentrations of sucrose in serum. LC/MS offered the advantage of high sensitivity and mass selectivity without the need for extensive sample derivatization required for GC/MS methods. However, the high polarity and non-volatile nature of the sucrose molecule renders LC/MS techniques challenging. Previously published reports lacked sufficient detail to permit replication of methodology. Problems encountered with existing protocols included poor peak resolution and weak fragmentation of the parent molecule. This communication describes a LC/MS protocol developed to provide improved resolution and product detection.

KW - Open access version available

KW - Liquid chromatography'mass spectrometry

KW - Peak resolution

KW - Sodiated sucrose

U2 - 10.1016/j.jchromb.2011.09.049

DO - 10.1016/j.jchromb.2011.09.049

M3 - Article

VL - 879

SP - 3668

EP - 3671

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 30

ER -