Development of a model for investigation of perennial ryegrass toxicosis in sheep

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AIMS: To develop a clinical model of perennial ryegrass toxicosis (PRGT) based on feeding a known dose of lolitrem B and ergotamine, and to produce a consistent clinical presentation for assessment of disease pathophysiology, neurological changes and neurohistopathology. METHODS: Male lambs, aged between 10–12 months, were randomly assigned to either Treatment (n=9) or Control (n=9) groups. Lambs in the Treatment group received feed containing a novel endophyte-infested perennial ryegrass seed, commencing on Day 0 of the Feeding phase with a low induction dose, then increasing after 3 days to provide 0.16 mg/kg live bodywight (LBW)/day of lolitrem B and 0.054 mg/kg LBW/day ergotamine. Lambs were examined daily and when defined signs of PRGT were observed they were transferred to the Testing phase. Neurological examinations, assessment of gait, surface electromyography (EMG) and mechanosensory nociceptive threshold testing were carried out and blood samples collected during both phases of the trial, with a full necropsy, histopathological examination and measurement of faecal cortisol metabolites (FCM) performed on Day 2 of the Testing phase.
Original languageEnglish
Pages (from-to)281-289
Number of pages9
JournalNew Zealand Veterinary Journal
Issue number6
Publication statusPublished - 02 Nov 2018

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  • Activities

    • 1 Internal HDR Supervision

    Clinical and Pathological Characterisation of Perennial Ryegrass Toxicosis and Investigation of Bromide as a Therapeutic Agent.

    Jane Quinn (Supervisor), Scott Edwards (Supervisor), Shane Raidal (Supervisor) & Adam Hamlin (Supervisor)

    01 Feb 201031 Aug 2020

    Activity: Supervision/Examination/MentoringInternal HDR Supervision

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