Development of a model for investigation of perennial ryegrass toxicosis in sheep

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

AIMS: To develop a clinical model of perennial ryegrass toxicosis (PRGT) based on feeding a known dose of lolitrem B and ergotamine, and to produce a consistent clinical presentation for assessment of disease pathophysiology, neurological changes and neurohistopathology. METHODS: Male lambs, aged between 10–12 months, were randomly assigned to either Treatment (n=9) or Control (n=9) groups. Lambs in the Treatment group received feed containing a novel endophyte-infested perennial ryegrass seed, commencing on Day 0 of the Feeding phase with a low induction dose, then increasing after 3 days to provide 0.16 mg/kg live bodywight (LBW)/day of lolitrem B and 0.054 mg/kg LBW/day ergotamine. Lambs were examined daily and when defined signs of PRGT were observed they were transferred to the Testing phase. Neurological examinations, assessment of gait, surface electromyography (EMG) and mechanosensory nociceptive threshold testing were carried out and blood samples collected during both phases of the trial, with a full necropsy, histopathological examination and measurement of faecal cortisol metabolites (FCM) performed on Day 2 of the Testing phase.
Original languageEnglish
Pages (from-to)281-289
Number of pages9
JournalNew Zealand Veterinary Journal
Volume66
Issue number6
DOIs
Publication statusPublished - 02 Nov 2018

Fingerprint

Lolium
ergotamine
Lolium perenne
Ergotamine
poisoning
Sheep
lambs
sheep
Endophytes
electromyography
testing
Neurologic Examination
Electromyography
gait
dosage
pathophysiology
endophytes
Gait
cortisol
Hydrocortisone

Cite this

@article{f884805a52d94e8587ea7bce4d6e623a,
title = "Development of a model for investigation of perennial ryegrass toxicosis in sheep",
abstract = "AIMS: To develop a clinical model of perennial ryegrass toxicosis (PRGT) based on feeding a known dose of lolitrem B and ergotamine, and to produce a consistent clinical presentation for assessment of disease pathophysiology, neurological changes and neurohistopathology. METHODS: Male lambs, aged between 10–12 months, were randomly assigned to either Treatment (n=9) or Control (n=9) groups. Lambs in the Treatment group received feed containing a novel endophyte-infested perennial ryegrass seed, commencing on Day 0 of the Feeding phase with a low induction dose, then increasing after 3 days to provide 0.16 mg/kg live bodywight (LBW)/day of lolitrem B and 0.054 mg/kg LBW/day ergotamine. Lambs were examined daily and when defined signs of PRGT were observed they were transferred to the Testing phase. Neurological examinations, assessment of gait, surface electromyography (EMG) and mechanosensory nociceptive threshold testing were carried out and blood samples collected during both phases of the trial, with a full necropsy, histopathological examination and measurement of faecal cortisol metabolites (FCM) performed on Day 2 of the Testing phase.",
keywords = "Perennial ryegrass, sheep, Bromide, Staggers, Treatment and management, ataxia, faecal cortisol metabolites, lolitrem B, Perennial ryegrass toxicosis, tremor",
author = "Martin Combs and Scott Edwards and Joshua Scherpenhuizen and Edward Narayan and A.E. Kessell and John Piltz and Shane Raidal and Julie Ramsay and Jane Quinn",
year = "2018",
month = "11",
day = "2",
doi = "10.1080/00480169.2018.1492986",
language = "English",
volume = "66",
pages = "281--289",
journal = "New Zealand Veterinary Journal",
issn = "0048-0169",
publisher = "New Zealand Veterinary Association",
number = "6",

}

TY - JOUR

T1 - Development of a model for investigation of perennial ryegrass toxicosis in sheep

AU - Combs, Martin

AU - Edwards, Scott

AU - Scherpenhuizen, Joshua

AU - Narayan, Edward

AU - Kessell, A.E.

AU - Piltz, John

AU - Raidal, Shane

AU - Ramsay, Julie

AU - Quinn, Jane

PY - 2018/11/2

Y1 - 2018/11/2

N2 - AIMS: To develop a clinical model of perennial ryegrass toxicosis (PRGT) based on feeding a known dose of lolitrem B and ergotamine, and to produce a consistent clinical presentation for assessment of disease pathophysiology, neurological changes and neurohistopathology. METHODS: Male lambs, aged between 10–12 months, were randomly assigned to either Treatment (n=9) or Control (n=9) groups. Lambs in the Treatment group received feed containing a novel endophyte-infested perennial ryegrass seed, commencing on Day 0 of the Feeding phase with a low induction dose, then increasing after 3 days to provide 0.16 mg/kg live bodywight (LBW)/day of lolitrem B and 0.054 mg/kg LBW/day ergotamine. Lambs were examined daily and when defined signs of PRGT were observed they were transferred to the Testing phase. Neurological examinations, assessment of gait, surface electromyography (EMG) and mechanosensory nociceptive threshold testing were carried out and blood samples collected during both phases of the trial, with a full necropsy, histopathological examination and measurement of faecal cortisol metabolites (FCM) performed on Day 2 of the Testing phase.

AB - AIMS: To develop a clinical model of perennial ryegrass toxicosis (PRGT) based on feeding a known dose of lolitrem B and ergotamine, and to produce a consistent clinical presentation for assessment of disease pathophysiology, neurological changes and neurohistopathology. METHODS: Male lambs, aged between 10–12 months, were randomly assigned to either Treatment (n=9) or Control (n=9) groups. Lambs in the Treatment group received feed containing a novel endophyte-infested perennial ryegrass seed, commencing on Day 0 of the Feeding phase with a low induction dose, then increasing after 3 days to provide 0.16 mg/kg live bodywight (LBW)/day of lolitrem B and 0.054 mg/kg LBW/day ergotamine. Lambs were examined daily and when defined signs of PRGT were observed they were transferred to the Testing phase. Neurological examinations, assessment of gait, surface electromyography (EMG) and mechanosensory nociceptive threshold testing were carried out and blood samples collected during both phases of the trial, with a full necropsy, histopathological examination and measurement of faecal cortisol metabolites (FCM) performed on Day 2 of the Testing phase.

KW - Perennial ryegrass

KW - sheep

KW - Bromide

KW - Staggers

KW - Treatment and management

KW - ataxia

KW - faecal cortisol metabolites

KW - lolitrem B

KW - Perennial ryegrass toxicosis

KW - tremor

UR - http://www.scopus.com/inward/record.url?scp=85050267507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050267507&partnerID=8YFLogxK

UR - https://www.tandfonline.com/toc/tnzv20/66/4?nav=tocList

U2 - 10.1080/00480169.2018.1492986

DO - 10.1080/00480169.2018.1492986

M3 - Article

C2 - 29949720

AN - SCOPUS:85050267507

VL - 66

SP - 281

EP - 289

JO - New Zealand Veterinary Journal

JF - New Zealand Veterinary Journal

SN - 0048-0169

IS - 6

ER -