Pakistan’ın Subtropikal Bölgelerinde Sığır Hypodermozisinin (Hypoderma lineatum) Tespit Edilmesinde İndirek ELİSA Yönteminin Kullanılması

Translated title of the contribution: Development of indirect ELISA for the diagnosis of Bovine Hypodermosis (Hypoderma lineatum) in the cattle of subtropical region of Pakistan

Haroon Ahmed, Rosario Panadero-Fontán, Ceferino López Sández, Mobushir Riaz Khan, Saira Asif, Irfan Mustaf, Abdul Ghani, Iftikhar Ahmad, Syed Muhammad Saqlan Naqvi, Mazhar Qayyum

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
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Abstract

The aim of this study was to develop an indirect ELISA for the detection of Hypoderma lineatum antibodies and to determine the influence of seroepidemiological factors on the seroprevalence of bovine hypodermosis in cattle of subtropical region of Pakistan. For this purpose a total of 1000 blood samples were taken from twenty eight villages of Rawalpindi, Attock, Chakwal and Jhelum districts. First instar larvae (L1) of the warble fy were collected from the surrounding abattoirs to obtain the antigen (HyC) used for development of indirect ELISA. The seroprevalence was 17.4% (174/1000). The highest seroprevalence was recorded in the Rawalpindi district (28.81%), followed by Attock (21.51%), Jhelum (10%) and Chakwal (6.66%). In present study the sensitivity and specificity was 97.28% and 96.44%, respectively. The epidemiological factors showed that the village having hilly locations, local breed, young animals, water bodies, extensive grazing, primary exposed and non medicated were more seropositive as compare to others (P<0.05). In conclusion, indirect ELISA developed during this study is very useful tool for early detection of bovine hypodermosis in cattle grazing in subtropical region of Pakistan.

Translated title of the contributionDevelopment of indirect ELISA for the diagnosis of Bovine Hypodermosis (Hypoderma lineatum) in the cattle of subtropical region of Pakistan
Original languageUndefined/Unknown
Pages (from-to)1017-1022
Number of pages6
JournalKafkas Universitesi Veteriner Fakultesi Dergisi
Volume19
Issue number6
Publication statusPublished - Nov 2013

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