TY - JOUR
T1 - Development of rHA1-ELISA for specific and sensitive detection of H9 subtype influenza virus
AU - Shahsavandi, Shahla
AU - Salmanian, Ali Hatef
AU - Ghorashi, Seyed Ali
AU - Masoudi, Shahin
AU - Fotouhi, Fatemeh
AU - Ebrahimi, Mohammad Majid
PY - 2011/1/1
Y1 - 2011/1/1
N2 - A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA1 subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples.
AB - A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA1 subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples.
KW - Avian influenza virus
KW - Baculoviruses
KW - Recombinant hemagglutinin
KW - RHA1-ELISA
UR - http://www.scopus.com/inward/record.url?scp=78650526996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650526996&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2010.11.012
DO - 10.1016/j.jviromet.2010.11.012
M3 - Article
C2 - 21111002
AN - SCOPUS:78650526996
VL - 171
SP - 260
EP - 263
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 1
ER -