Development of rHA1-ELISA for specific and sensitive detection of H9 subtype influenza virus

Shahla Shahsavandi; Ali Hatef Salmanian; Seyed Ali Ghorashi; Shahin Masoudi; Fatemeh Fotouhi; Mohammad Majid Ebrahimi

doi:10.1016/j.jviromet.2010.11.012

Development of rHA1-ELISA for specific and sensitive detection of H9 subtype influenza virus

Shahla Shahsavandi, Ali Hatef Salmanian, Seyed Ali Ghorashi, Shahin Masoudi, Fatemeh Fotouhi, Mohammad Majid Ebrahimi

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA₁ subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples.

Original language	English
Pages (from-to)	260-263
Number of pages	4
Journal	Journal of Virological Methods
Volume	171
Issue number	1
DOIs	https://doi.org/10.1016/j.jviromet.2010.11.012
Publication status	Published - 01 Jan 2011

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title = "Development of rHA1-ELISA for specific and sensitive detection of H9 subtype influenza virus",

abstract = "A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA1 subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples.",

keywords = "Avian influenza virus, Baculoviruses, Recombinant hemagglutinin, RHA1-ELISA",

author = "Shahla Shahsavandi and Salmanian, {Ali Hatef} and Ghorashi, {Seyed Ali} and Shahin Masoudi and Fatemeh Fotouhi and Ebrahimi, {Mohammad Majid}",

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AU - Shahsavandi, Shahla

AU - Salmanian, Ali Hatef

AU - Ghorashi, Seyed Ali

AU - Masoudi, Shahin

AU - Fotouhi, Fatemeh

AU - Ebrahimi, Mohammad Majid

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