Abstract
Objectives: The objective of this study was to chemically identify and quantify structural changes in porcine milk oligosaccharides (PMOs) during lactation in pigs in their first and multiple pregnancies.
Methods: 2 to 5 mL of milk sample at 3 time-point of lactation (colostrum at day 1, transitional milk at day 3 and mature milk at day 15 of lactation) was collected from each gilt (n=7) and sow (n=10). The oligosaccharide fractions form the milk was separated using solvent extraction method (chloroform/methanol -2:1) which were then analysed using Isotope-labelling associated LC-ESI-MS/MS.
Results: We reported 55 PMOs structures, of which 25 are new (17 sialylated and 8 neutral). Their incidence in gilt and sow colostrum was almost identical (53 vs. 54), but not in transition milk (48 vs. 53) nor mature milk (41 vs. 47). These PMOs including neutral-, sialyl- and fucosyl- MOs in colostrum were more abundant in the gilt than the sow, but always decreased during lactation. Structural diversity also decreased, although fucosylated MO were conserved.
Conclusion: Our findings infer a key mechanism influencing the observed phenotypes of sow produces significantly more milk and had a more successful pregnancy outcome than gilt in pig reproductions and will assist in the future identification of mechanisms responsible for more successful pregnancy outcomes in women with second babies.
Methods: 2 to 5 mL of milk sample at 3 time-point of lactation (colostrum at day 1, transitional milk at day 3 and mature milk at day 15 of lactation) was collected from each gilt (n=7) and sow (n=10). The oligosaccharide fractions form the milk was separated using solvent extraction method (chloroform/methanol -2:1) which were then analysed using Isotope-labelling associated LC-ESI-MS/MS.
Results: We reported 55 PMOs structures, of which 25 are new (17 sialylated and 8 neutral). Their incidence in gilt and sow colostrum was almost identical (53 vs. 54), but not in transition milk (48 vs. 53) nor mature milk (41 vs. 47). These PMOs including neutral-, sialyl- and fucosyl- MOs in colostrum were more abundant in the gilt than the sow, but always decreased during lactation. Structural diversity also decreased, although fucosylated MO were conserved.
Conclusion: Our findings infer a key mechanism influencing the observed phenotypes of sow produces significantly more milk and had a more successful pregnancy outcome than gilt in pig reproductions and will assist in the future identification of mechanisms responsible for more successful pregnancy outcomes in women with second babies.
| Original language | English |
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| Number of pages | 1 |
| Publication status | Published - 2018 |
| Event | Nutrition 2018: American Society for Nutrition annual meeting - Hynes Convention Centre, Boston, United States Duration: 09 Jun 2018 → 12 Jun 2018 https://www.nutritionsociety.org/events/nutrition-2018 (Conference website) |
Conference
| Conference | Nutrition 2018 |
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| Country/Territory | United States |
| City | Boston |
| Period | 09/06/18 → 12/06/18 |
| Other | The American Society for Nutrition’s new scientific sessions and annual meeting will convene in Boston to focus on the multidisciplinary field of nutrition science. It will bring together basic, translational, clinical, and population scientists and practitioners. Expect interactive exhibits, new technology, and an immersive learning experience. At Nutrition 2018, the top scientific researchers, practitioners, global and public health professionals, policy makers and advocacy leaders, industry, media and other related professionals will gather to advance nutrition science and its practical application. |
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