Developmental and structural changes in porcine milk oligosaccharies during lactation in first and multiple pregnancies

A HPLC-ESI-MS/MS study

Bing Wang, Jinhua Wei, Zhuo Wang, Marefa Jahan, Zhongfu Wang, Peter Wynn, Yuguang Du

Research output: Other contribution to conferencePoster

Abstract

Objectives: The objective of this study was to chemically identify and quantify structural changes in porcine milk oligosaccharides (PMOs) during lactation in pigs in their first and multiple pregnancies.
Methods: 2 to 5 mL of milk sample at 3 time-point of lactation (colostrum at day 1, transitional milk at day 3 and mature milk at day 15 of lactation) was collected from each gilt (n=7) and sow (n=10). The oligosaccharide fractions form the milk was separated using solvent extraction method (chloroform/methanol -2:1) which were then analysed using Isotope-labelling associated LC-ESI-MS/MS.
Results: We reported 55 PMOs structures, of which 25 are new (17 sialylated and 8 neutral). Their incidence in gilt and sow colostrum was almost identical (53 vs. 54), but not in transition milk (48 vs. 53) nor mature milk (41 vs. 47). These PMOs including neutral-, sialyl- and fucosyl- MOs in colostrum were more abundant in the gilt than the sow, but always decreased during lactation. Structural diversity also decreased, although fucosylated MO were conserved.
Conclusion: Our findings infer a key mechanism influencing the observed phenotypes of sow produces significantly more milk and had a more successful pregnancy outcome than gilt in pig reproductions and will assist in the future identification of mechanisms responsible for more successful pregnancy outcomes in women with second babies.
Original languageEnglish
Number of pages1
Publication statusPublished - 2018
EventNutrition 2018: American Society for Nutrition annual meeting - Hynes Convention Centre, Boston, United States
Duration: 09 Jun 201812 Jun 2018
https://www.nutritionsociety.org/events/nutrition-2018 (Conference website)

Conference

ConferenceNutrition 2018
CountryUnited States
CityBoston
Period09/06/1812/06/18
OtherThe American Society for Nutrition’s new scientific sessions and annual meeting will convene in Boston to focus on the multidisciplinary field of nutrition science. It will bring together basic, translational, clinical, and population scientists and practitioners. Expect interactive exhibits, new technology, and an immersive learning experience.
At Nutrition 2018, the top scientific researchers, practitioners, global and public health professionals, policy makers and advocacy leaders, industry, media and other related professionals will gather to advance nutrition science and its practical application.
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lactation
pregnancy
milk
swine
gilts
oligosaccharides
sows
colostrum
pregnancy outcome
electrospray ionization mass spectrometry
tandem mass spectrometry
isotope labeling
infants
chloroform
methanol
phenotype
incidence
methodology

Cite this

Wang, B., Wei, J., Wang, Z., Jahan, M., Wang, Z., Wynn, P., & Du, Y. (2018). Developmental and structural changes in porcine milk oligosaccharies during lactation in first and multiple pregnancies: A HPLC-ESI-MS/MS study. Poster session presented at Nutrition 2018, Boston, United States.
Wang, Bing ; Wei, Jinhua ; Wang, Zhuo ; Jahan, Marefa ; Wang, Zhongfu ; Wynn, Peter ; Du, Yuguang. / Developmental and structural changes in porcine milk oligosaccharies during lactation in first and multiple pregnancies : A HPLC-ESI-MS/MS study. Poster session presented at Nutrition 2018, Boston, United States.1 p.
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abstract = "Objectives: The objective of this study was to chemically identify and quantify structural changes in porcine milk oligosaccharides (PMOs) during lactation in pigs in their first and multiple pregnancies.Methods: 2 to 5 mL of milk sample at 3 time-point of lactation (colostrum at day 1, transitional milk at day 3 and mature milk at day 15 of lactation) was collected from each gilt (n=7) and sow (n=10). The oligosaccharide fractions form the milk was separated using solvent extraction method (chloroform/methanol -2:1) which were then analysed using Isotope-labelling associated LC-ESI-MS/MS.Results: We reported 55 PMOs structures, of which 25 are new (17 sialylated and 8 neutral). Their incidence in gilt and sow colostrum was almost identical (53 vs. 54), but not in transition milk (48 vs. 53) nor mature milk (41 vs. 47). These PMOs including neutral-, sialyl- and fucosyl- MOs in colostrum were more abundant in the gilt than the sow, but always decreased during lactation. Structural diversity also decreased, although fucosylated MO were conserved.Conclusion: Our findings infer a key mechanism influencing the observed phenotypes of sow produces significantly more milk and had a more successful pregnancy outcome than gilt in pig reproductions and will assist in the future identification of mechanisms responsible for more successful pregnancy outcomes in women with second babies.",
author = "Bing Wang and Jinhua Wei and Zhuo Wang and Marefa Jahan and Zhongfu Wang and Peter Wynn and Yuguang Du",
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Developmental and structural changes in porcine milk oligosaccharies during lactation in first and multiple pregnancies : A HPLC-ESI-MS/MS study. / Wang, Bing; Wei, Jinhua; Wang, Zhuo; Jahan, Marefa; Wang, Zhongfu ; Wynn, Peter; Du, Yuguang.

2018. Poster session presented at Nutrition 2018, Boston, United States.

Research output: Other contribution to conferencePoster

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T1 - Developmental and structural changes in porcine milk oligosaccharies during lactation in first and multiple pregnancies

T2 - A HPLC-ESI-MS/MS study

AU - Wang, Bing

AU - Wei, Jinhua

AU - Wang, Zhuo

AU - Jahan, Marefa

AU - Wang, Zhongfu

AU - Wynn, Peter

AU - Du, Yuguang

N1 - P11-013. Poster Topical Area Experimental Animal Nutrition

PY - 2018

Y1 - 2018

N2 - Objectives: The objective of this study was to chemically identify and quantify structural changes in porcine milk oligosaccharides (PMOs) during lactation in pigs in their first and multiple pregnancies.Methods: 2 to 5 mL of milk sample at 3 time-point of lactation (colostrum at day 1, transitional milk at day 3 and mature milk at day 15 of lactation) was collected from each gilt (n=7) and sow (n=10). The oligosaccharide fractions form the milk was separated using solvent extraction method (chloroform/methanol -2:1) which were then analysed using Isotope-labelling associated LC-ESI-MS/MS.Results: We reported 55 PMOs structures, of which 25 are new (17 sialylated and 8 neutral). Their incidence in gilt and sow colostrum was almost identical (53 vs. 54), but not in transition milk (48 vs. 53) nor mature milk (41 vs. 47). These PMOs including neutral-, sialyl- and fucosyl- MOs in colostrum were more abundant in the gilt than the sow, but always decreased during lactation. Structural diversity also decreased, although fucosylated MO were conserved.Conclusion: Our findings infer a key mechanism influencing the observed phenotypes of sow produces significantly more milk and had a more successful pregnancy outcome than gilt in pig reproductions and will assist in the future identification of mechanisms responsible for more successful pregnancy outcomes in women with second babies.

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