Dietary protein source significantly alters growth performance, plasma variables and hepatic gene expression in rainbow trout (Oncorhynchus mykiss) fed amino acid balanced diets

Jurij Wacyk, Madison Powell, Kenneth Rodnick, Ken Overturf, R. A. Hill, Ronald Hardy

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The objective of the study was to evaluate the effect of dietary protein source on fish growth, nutrient utilization, plasma variables and hepatic gene expression in juvenile rainbow trout (Oncorhynchus mykiss). Fishmeal (FM) and soy protein isolate (SPI) were used as the main sources of protein in six isonitrogenous, isolipidic and isocaloric diets. The amino acid profiles of the diets were completely balanced to minimize differences between experimental treatments and formulated to contain increasing levels of branched-chain amino acids (BCAA) based upon dietary requirements for trout (NRC, 1993). Dietary protein source more consistently changed the measured variables while BCAA supplementation had an unexpected effect over whole body lipid content. Growth performance and protein retention efficiency were significantly reduced in fish fed SPI diets independently of BCAA supplementation. Total concentration of amino acids as well as circulating indispensable amino acids (IAA) were significantly elevated in the plasma of fish receiving SPI diets compared to fish fed FM diets. The change in IAAwas large enough to increase (p
Original languageEnglish
Pages (from-to)223-234
Number of pages12
JournalAquaculture
Volume356
Early online dateMay 2012
DOIs
Publication statusPublished - Aug 2012

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protein sources
rainbow
dietary protein
gene expression
Oncorhynchus mykiss
growth performance
amino acid
branched chain amino acids
soy protein isolate
diet
plasma
liver
amino acids
protein
fish meal
fish feeds
fish
nutrient utilization
trout
body fat

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title = "Dietary protein source significantly alters growth performance, plasma variables and hepatic gene expression in rainbow trout (Oncorhynchus mykiss) fed amino acid balanced diets",
abstract = "The objective of the study was to evaluate the effect of dietary protein source on fish growth, nutrient utilization, plasma variables and hepatic gene expression in juvenile rainbow trout (Oncorhynchus mykiss). Fishmeal (FM) and soy protein isolate (SPI) were used as the main sources of protein in six isonitrogenous, isolipidic and isocaloric diets. The amino acid profiles of the diets were completely balanced to minimize differences between experimental treatments and formulated to contain increasing levels of branched-chain amino acids (BCAA) based upon dietary requirements for trout (NRC, 1993). Dietary protein source more consistently changed the measured variables while BCAA supplementation had an unexpected effect over whole body lipid content. Growth performance and protein retention efficiency were significantly reduced in fish fed SPI diets independently of BCAA supplementation. Total concentration of amino acids as well as circulating indispensable amino acids (IAA) were significantly elevated in the plasma of fish receiving SPI diets compared to fish fed FM diets. The change in IAAwas large enough to increase (p",
author = "Jurij Wacyk and Madison Powell and Kenneth Rodnick and Ken Overturf and Hill, {R. A.} and Ronald Hardy",
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Dietary protein source significantly alters growth performance, plasma variables and hepatic gene expression in rainbow trout (Oncorhynchus mykiss) fed amino acid balanced diets. / Wacyk, Jurij; Powell, Madison; Rodnick, Kenneth; Overturf, Ken; Hill, R. A.; Hardy, Ronald.

In: Aquaculture, Vol. 356, 08.2012, p. 223-234.

Research output: Contribution to journalArticle

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