Differentiation between pathogenic serotype 1 isolates of Marek's disease virus (MDV1) and the Rispens vaccine in Australia using real-time PCR.

K.G. Renz, B.F. Cheethan, S.W. Walkden-Brown

Research output: Book chapter/Published conference paperConference paper

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Abstract

Two real-time PCR assays were developed which enable quantification and differentiation between pathogenic Australian isolates of MDV serotype 1 and the serotype 1 vaccine strain Rispens/CVI988. The assays are based on a DNA sequence variation in the meq gene between pathogenic and vaccinal MDV1 which has been confirmed by sequencing of 20 Australian field strains of MDV. Complete specificity has been demonstrated in samples containing pathogenic MDV (n=20), Rispens (3 commercial vaccine strains), or both. The limit of detection of both the Rispens-specific and the pathogenic MDV1-specific assays was 10 viral copies/reaction.
Original languageEnglish
Title of host publication21st Annual Proceedings
EditorsP. Selle
Place of PublicationAustralia
PublisherUniversity of Sydney
Pages107-110
Number of pages4
Publication statusPublished - 2010
EventAustralian Poultry Science Symposium - Sydney, NSW Australia, Australia
Duration: 01 Feb 201003 Feb 2010

Conference

ConferenceAustralian Poultry Science Symposium
CountryAustralia
Period01/02/1003/02/10

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    Renz, K. G., Cheethan, B. F., & Walkden-Brown, S. W. (2010). Differentiation between pathogenic serotype 1 isolates of Marek's disease virus (MDV1) and the Rispens vaccine in Australia using real-time PCR. In P. Selle (Ed.), 21st Annual Proceedings (pp. 107-110). University of Sydney.