Direct detection of vanB2 using the Roche LightCycler vanA/B detection assay to indicate vancomycin-resistant enterococcal carriage: sensitive but not specific

H.L. Young, S.A. Ballard, P Roffey, M.L. Grayson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Roche LightCycler VRE Detection Kit (Roche Diagnostics, Mannheim, Germany) has been developed to rapidly assess for VRE colonization. 3 This kit provides the primers and the hybridization probes for the amplification and sequence-specific detection of vanA, vanB1 and vanB2/3, along with an internal control for detection of sample inhibition. It has a processing time of approximately 1.5 h. Using this kit with DNA directly extracted from clinical specimens, detection of VRE can be reduced to less than 24 h. However, when used in this manner, it is important to note that the system detects van genes rather than VRE per se.
Original languageEnglish
Pages (from-to)809-810
Number of pages2
JournalJournal of Antimicrobial Chemotherapy
Volume59
Issue number4
DOIs
Publication statusPublished - 2007
Externally publishedYes

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