Dothiorella and Spencermartinsia are two Botryosphaeriaceous genera found on various woody hosts that bear two-celled conidia that become brown and 1-septate prior to dehiscence from conidiogenous cells. Based on morphological characters and phylogeny of DNA sequences of the internal transcribed spacer (ITS) region and part of the translation elongation factor (TEF) 1–α gene, three new species, namely Spencermartinsia westrale W.M. Pitt, J.R. Úrbez-Torres & Trouillas, Dothiorella neclivorem W.M. Pitt & J.R. Úrbez-Torres and Dothiorella vinea-gemmae W.M. Pitt & J.R. Úrbez-Torres are described from grapevines in Australia. Additionally, Spencermartinsia plurivora Abdollahz., Javadi & A.J.L. Phillips, and a Dothiorella species, currently unnamed but previously isolated from walnut (Juglans regia L.) in France and Iran and hop hornbeam (Ostrya carpinifolia Scop.) in Italy, are reported for the first time in Australia, the latter also for the first time from grapevines.