Strains of a coelomycete isolated from grapevinecankers in southeastern Australia and identified as Dothiorellaiberica in previous studies are redescribed in this study as anovel species based on morphological characters and phylogeneticanalyses of DNA sequences of the internal transcribedspacer region (ITS1-5.8S-ITS2), and partial sequences of thetranslation elongation factor 1-ÃŽ± and ÃŽÂ²-tubulin genes.Dothiorella vidmadera sp. nov. is most closely related to D.iberica, D. americana and D. sarmentorum, but differs inmorphological characters and DNA sequences. All four speciesare known to be associated with Botryosphaeria diebackof grapevines. Additionally, an unidentified ascomycete isolatedfrom dead wood of grapevines in Western Australia isdescribed. Phylogenetically, strains were most closely relatedto Spencermartinsia viticola, and bore conidia with morphologicalcharacters and dimensions consistent with publisheddescriptions. However, ascospores were shorter and narrowerthan previously reported and lacked the terminal apiculi thattypify the genus.