Effect of β3-Adrenergic Eeceptor on Atrial L-type Ca2+ current in rats with chronic heart failure

Biao Li, Qiang Zhao, Lexin Wang, Chun-Yu Deng, Fang Zeng, Tong-Guo Wu, Zuo-Feng Jiang, Ding-Li Xu

Research output: Contribution to journalArticle

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Abstract

Objectives: To investigate the effect of selective ß3-adrenoreceptor agonist BRL-37344 on L-type Ca2+ current (Ica,L) and mRNA expression of L-type Ca2+ channel a2d-2 (Cacna2d2) in rats with chronic heart failure (CHF).  Methods: Twenty-four male Wistar rats were divided into normal control (n= 6) and CHF group (n= 18), which were further divided into CHF control and BRL group (0.4. nmol/kg, IV, twice weekly for four weeks). Echocardiography was performed to assess the structure and function of the left atrium (LA). Results: The LA in the BRL group (4.4±0.2mm) was larger than in the normal control (3.5±0.3mm, P<0.01) or CHF control (4.0±0.2mm, P<0.05) group. The LA ejection fraction in the BRL group (36.2±4.2%) was lower than in the normal control (58.0±3.1%, P<0.01) or CHF control group (42.3±4.8%, P<0.05). There was no difference in Ica,L density between the BRL group and CHF control group (8.3±1.7 vs. 8.2±2.6pA/pF, P>0.05), which was higher than in the normal control group (6.0±1.8pA/pF, P<0.01). There was no difference in the mRNA expression of a2d-2 (Cacna2d2) between the BRL group and CHF control group (0.264±0.005 vs. 0.243±0.017, P>0.05), which was also higher than in the normal control group (0.137±0.013, P<0.01).  Conclusion: ß3-Adrenoreceptor stimulation with BRL-37344 was associated with an increase in LA diameter and a decrease in LA function in chronic heart failure. These structural and function changes were not related to Ica,L or L-type Ca2+ channel a2d-2 (Cacna2d2) subunit in the LA myocytes.
Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalHeart Lung and Circulation
Volume23
Issue number4
DOIs
Publication statusPublished - Apr 2014

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