Effect of atmospheric cold plasma on structure, activity, and reversible assembly of the phytoferritin

Rui Yang, Yuqian Liu, Demei Meng, Desheng Wang, Christopher L. Blanchard, Zhongkai Zhou

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ferritin is characterized by a shell-like structure and a reversible self-assembly property. In this study, atmospheric cold plasma (ACP) was applied to red bean seed ferritin (RBF) to prepare an ACP-treated RBF (ACPF). Results indicated that the ACP treatment retained the shell-like structure of ferritin but reduced the α-helix/β-sheet contents and thermal stability. Iron oxidative deposition and release activities were also markedly changed. The ACPF could be disassembled at pH 4.0 and then assembled into an intact ferritin cage when pH was increased to 7.0, which was a more benign transition condition than that of the traditional method (pH 2.0/7.0 transition). By using this assembly routine, curcumin was successfully encapsulated within the ACPF with a size distribution of 12 nm. Moreover, the encapsulation ratio of curcumin in the ACPF reached 12.7% (w/w). This finding can be used to expand the application of ACP and improve the functionalization of the ferritin.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalFood Chemistry
Volume264
Early online date16 Apr 2018
DOIs
Publication statusPublished - 30 Oct 2018

Fingerprint

Plasma Gases
ferritin
Ferritins
red beans
Curcumin
curcumin
Seed
Seeds
encapsulation
thermal stability
seeds
Encapsulation
Self assembly
phytoferritin
cages
Thermodynamic stability
Iron
Hot Temperature
iron

Cite this

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title = "Effect of atmospheric cold plasma on structure, activity, and reversible assembly of the phytoferritin",
abstract = "Ferritin is characterized by a shell-like structure and a reversible self-assembly property. In this study, atmospheric cold plasma (ACP) was applied to red bean seed ferritin (RBF) to prepare an ACP-treated RBF (ACPF). Results indicated that the ACP treatment retained the shell-like structure of ferritin but reduced the α-helix/β-sheet contents and thermal stability. Iron oxidative deposition and release activities were also markedly changed. The ACPF could be disassembled at pH 4.0 and then assembled into an intact ferritin cage when pH was increased to 7.0, which was a more benign transition condition than that of the traditional method (pH 2.0/7.0 transition). By using this assembly routine, curcumin was successfully encapsulated within the ACPF with a size distribution of 12 nm. Moreover, the encapsulation ratio of curcumin in the ACPF reached 12.7{\%} (w/w). This finding can be used to expand the application of ACP and improve the functionalization of the ferritin.",
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Effect of atmospheric cold plasma on structure, activity, and reversible assembly of the phytoferritin. / Yang, Rui; Liu, Yuqian; Meng, Demei; Wang, Desheng; Blanchard, Christopher L.; Zhou, Zhongkai.

In: Food Chemistry, Vol. 264, 30.10.2018, p. 41-48.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of atmospheric cold plasma on structure, activity, and reversible assembly of the phytoferritin

AU - Yang, Rui

AU - Liu, Yuqian

AU - Meng, Demei

AU - Wang, Desheng

AU - Blanchard, Christopher L.

AU - Zhou, Zhongkai

PY - 2018/10/30

Y1 - 2018/10/30

N2 - Ferritin is characterized by a shell-like structure and a reversible self-assembly property. In this study, atmospheric cold plasma (ACP) was applied to red bean seed ferritin (RBF) to prepare an ACP-treated RBF (ACPF). Results indicated that the ACP treatment retained the shell-like structure of ferritin but reduced the α-helix/β-sheet contents and thermal stability. Iron oxidative deposition and release activities were also markedly changed. The ACPF could be disassembled at pH 4.0 and then assembled into an intact ferritin cage when pH was increased to 7.0, which was a more benign transition condition than that of the traditional method (pH 2.0/7.0 transition). By using this assembly routine, curcumin was successfully encapsulated within the ACPF with a size distribution of 12 nm. Moreover, the encapsulation ratio of curcumin in the ACPF reached 12.7% (w/w). This finding can be used to expand the application of ACP and improve the functionalization of the ferritin.

AB - Ferritin is characterized by a shell-like structure and a reversible self-assembly property. In this study, atmospheric cold plasma (ACP) was applied to red bean seed ferritin (RBF) to prepare an ACP-treated RBF (ACPF). Results indicated that the ACP treatment retained the shell-like structure of ferritin but reduced the α-helix/β-sheet contents and thermal stability. Iron oxidative deposition and release activities were also markedly changed. The ACPF could be disassembled at pH 4.0 and then assembled into an intact ferritin cage when pH was increased to 7.0, which was a more benign transition condition than that of the traditional method (pH 2.0/7.0 transition). By using this assembly routine, curcumin was successfully encapsulated within the ACPF with a size distribution of 12 nm. Moreover, the encapsulation ratio of curcumin in the ACPF reached 12.7% (w/w). This finding can be used to expand the application of ACP and improve the functionalization of the ferritin.

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