Effect of combined exposure of diethylphthalate and nonylphenolon on growth of Dunaliella salina

WANG Jing-jing, QIAN Xiao-jia, Min An, DUAN Shun-shan

Research output: Contribution to journalArticle

Abstract

Combined toxic effect of diethylphthalate (DEP) and nonylphenol (NP)on the growth of Dunaliella salina was evaluated bytaking the 96h EC50 as one toxic unit (TU). Cell density, protein content, chloroplast pigment content, SOD activity, and maximalphotochemical efficiency of PSII (Fv/Fm) of D. salina were examined after the single and three different combined exposure for 96h(toxic unit proportion 1:1,1:4, 4:1).The results showed that both DEP and NP inhabited the growth of D. salina. Moreover, the toxiceffect of simultaneous exposure was more significant than single exposure of the two chemicals. The values of 96h EC50 of DEP and NPon D. salina were 69.54 mg/L and 1.47 mg/L, respectively. The single toxic effect as well as combined toxic effect on the biomass,soluble protein content'chloroplast pigment'and SOD activity content of D. salina was detected based on the different toxicity unitproportions. Synergetic effect was observed for the toxicity of DEP and NP in the three toxicity unit proportions, which was moreobvious in the proportion of 1:1.
Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalEcological Science
Volume31
Issue number4
Publication statusPublished - Jul 2012

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