Combined toxic effect of diethylphthalate (DEP) and nonylphenol (NP)on the growth of Dunaliella salina was evaluated bytaking the 96h EC50 as one toxic unit (TU). Cell density, protein content, chloroplast pigment content, SOD activity, and maximalphotochemical efficiency of PSII (Fv/Fm) of D. salina were examined after the single and three different combined exposure for 96h(toxic unit proportion 1:1,1:4, 4:1).The results showed that both DEP and NP inhabited the growth of D. salina. Moreover, the toxiceffect of simultaneous exposure was more significant than single exposure of the two chemicals. The values of 96h EC50 of DEP and NPon D. salina were 69.54 mg/L and 1.47 mg/L, respectively. The single toxic effect as well as combined toxic effect on the biomass,soluble protein content'chloroplast pigment'and SOD activity content of D. salina was detected based on the different toxicity unitproportions. Synergetic effect was observed for the toxicity of DEP and NP in the three toxicity unit proportions, which was moreobvious in the proportion of 1:1.
|Number of pages||7|
|Publication status||Published - Jul 2012|