Effect of diabetic osteoblasts on osteogenic differentiation of human umbilical cord mesenchymal stem cells

Cheng-Zhi Ha, Hai-Ying Chen, Juan Wang, Wei Liu, Ying-Xin Zhang, Li Pan, Wei-Hua Wang, Shuang-Feng Chen, Da-Wei Wang, Lexin Wang

Research output: Contribution to journalArticlepeer-review

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Abstract

Background. This study was aimed to investigate whether osteoblasts from diabetic patients have a promoting effect on osteogenesis of human umbilical cord mesenchymal stem cells (HUMSCs). Methods. HUMSCs were co-cultured with osteoblasts of diabetic and non-diabetic patients. Morphological appear- ance and cytochemical characteristics of the non-diabetic osteoblasts and diabetic osteoblasts were observed by hematoxylin-eosin staining, type I collagen protein expression, alkaline phosphatase (ALP) staining and Alizarin Red S staining. Cell viability, type I collagen protein expression, ALP activity and osteocalcin mRNA expression in HUMSCs were investigated. Results. Compared with negative control group, the cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA were increased in HUMSCs co-cultured with diabetic and non-diabetic osteoblasts (P<0.05). There was no statistically significant difference in the HUMSCs cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA between the non-diabetic and diabetic group (P >0.05). Conclusions. Similar to osteoblasts from non-diabetic patients, osteoblasts from diabetic patients also have the ability to promote HUMSCs proliferation, and leading to HUMSCs exhibit some characteristic of osteoblasts.
Original languageEnglish
Pages (from-to)388-393
Number of pages6
JournalBiomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
Volume159
Issue number3
Early online date2014
DOIs
Publication statusPublished - 2015

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