TY - JOUR
T1 - Effect of exposure to mitomycin C on cultured tympanic membrane fibroblasts
AU - Jang, Chul Ho
AU - Song, Chang-Hun
AU - Pak, Sokcheon
N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = International Journal of Pediatric Otorhinolaryngology. ISSNs: 0165-5876;
PY - 2003
Y1 - 2003
N2 - Objective: Recently, attempts have been made to prolong the patency of myringotomy site with topical use of mitomycin C (MMC). It has been shown that MMC inhibits mitosis and proliferation of ocular fibroblasts, however, there are no studies of MMC's effect on tympanic membrane fibroblasts. To investigate the effects of MMC on cultured human tympanic membrane fibroblasts and understand the cellular basis of MMC for maintain myringotomy patency, cultured fibroblasts were exposed to various concentrations of MMC for periods of 5'10 min. Methods: Effect of MMC on cultured fibroblasts was assessed by microscopic observation and cell viability test. Results: Dose-, time- dependent relationship of MMC on cultured fibroblasts was revealed. There was a significant difference between the inhibition effects of MMC at concentrations of 0.4 mg/ml and control following 5 and 10 min exposure intervals. Phase-contrast microscopy showed consistency with the antiproliferative effect of MMC at higher concentration. Conclusions: Therefore, it would appear that intraoperative use of MMC could be effective in delaying the healing of the myringotomy site and extending the period of time for myringotomy patency.
AB - Objective: Recently, attempts have been made to prolong the patency of myringotomy site with topical use of mitomycin C (MMC). It has been shown that MMC inhibits mitosis and proliferation of ocular fibroblasts, however, there are no studies of MMC's effect on tympanic membrane fibroblasts. To investigate the effects of MMC on cultured human tympanic membrane fibroblasts and understand the cellular basis of MMC for maintain myringotomy patency, cultured fibroblasts were exposed to various concentrations of MMC for periods of 5'10 min. Methods: Effect of MMC on cultured fibroblasts was assessed by microscopic observation and cell viability test. Results: Dose-, time- dependent relationship of MMC on cultured fibroblasts was revealed. There was a significant difference between the inhibition effects of MMC at concentrations of 0.4 mg/ml and control following 5 and 10 min exposure intervals. Phase-contrast microscopy showed consistency with the antiproliferative effect of MMC at higher concentration. Conclusions: Therefore, it would appear that intraoperative use of MMC could be effective in delaying the healing of the myringotomy site and extending the period of time for myringotomy patency.
U2 - 10.1016/S0165-5876(02)00367-1
DO - 10.1016/S0165-5876(02)00367-1
M3 - Article
VL - 67
SP - 173
EP - 176
JO - International Journal of Pediatric Otorhinolaryngology
JF - International Journal of Pediatric Otorhinolaryngology
SN - 0165-5876
IS - 2
ER -