Effect of green tea extract (Camellia sinensis) on fertility indicators of post-thawed bull spermatozoa

Hamayun Khan, Momen Khan, Muhammad Subhan Qureshi, Shakoor Ahmad, Ali Gohar, Hameed Ullah, Farman Ullah, Arab Hussain, Pershotam Khatri, Said Sajjad Ali Shah, (Late) Hamid Rehman, Azmatullah Khan

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Green tea extract is plant–derived natural antioxidant. The objective of this study was to explore the antioxidative effect of green tea extract on the cryopreserved bull semen. Experiments were carried out on Achai breeding bulls of similar age at the Semen Production Unit, Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed spermatozoa motility, viability and membrane integrity of Achai bull.

Original languageEnglish
Pages (from-to)1243-1249
Number of pages7
JournalPakistan Journal of Zoology
Issue number4
Publication statusPublished - Aug 2017


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