Effect of isoflavone on balloon catheter-induced neointimal hyperplasia in rabbit carotid artery

G-Y. Zhang, R.-F Qiu, Y.-C Sun, Lexin Wang

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Abstract

Background: This study was designed to investigate the effects of phytoestrogen isoflavone on balloon catheter-induced hyperplasia of carotid artery. Methods: Forty-eight female New Zealand rabbits were randomly divided into four groups: control (balloon-induced carotid artery injury only); ovariectomy control (ovariectomy and carotid artery injury), oestrogen (ovariectomy, carotid artery injury and nilestriol, 5. mg/kg daily for 28 days), and isoflavone (ovariectomy, carotid artery injury and isoflavone 120. mg/kg daily for 28 days). The arterial wall thickness was assessed by coloured ultrasonography, and the oestrogen-' and oestrogen-ß receptors in the abdominal aorta were measured by Western blotting. Results: The medial layer thickness in the isoflavone group was less than in the ovariectomy control group (0.28 ± 0.03 vs. 0.35 ± 0.04. mm, p< 0.01), and the intimal/medial layer (I/. M) ratio is the isoflavone group was also less than in the ovariectomy control group (16.85 ± 3.79 vs. 48.94 ± 8.92, p< 0.01). There was no statistically significant difference in the medial layer thickness or I/. M ratio between the isoflavone and the oestrogen groups. The optical density of the oestrogen-' receptors in the isoflavone group (0.317 ± 0.002) was less than in the oestrogen (0.633 ± 0.002) or ovariectomy control group (0.590 ± 0.001, p< 0.01). The optical density of the oestrogen-ß receptors in the isoflavone group (1.350 ± 0.002) and the ovariectomy control group (1.2033 ± 0.002) was less than in the oestrogen group (1.7699 ± 0.003, p< 0.01). Conclusions: Isoflavone therapy in the ovariectomised rabbit model attenuated balloon catheter-induced intimal and medial layer hyperplasia in the carotid arteries. Down-regulation of the oestrogen-' receptors may be involved in the hyperplasia-preventative effect.
Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalHeart Lung and Circulation
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 2013

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Isoflavones
Carotid Arteries
Ovariectomy
Hyperplasia
Catheters
Rabbits
Carotid Artery Injuries
Estrogens
Estrogen Receptors
Control Groups
Tunica Intima
Phytoestrogens
Abdominal Aorta
Ultrasonography
Down-Regulation
Western Blotting

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@article{dd6b6c5173ee4ed3aa322574b157bdc3,
title = "Effect of isoflavone on balloon catheter-induced neointimal hyperplasia in rabbit carotid artery",
abstract = "Background: This study was designed to investigate the effects of phytoestrogen isoflavone on balloon catheter-induced hyperplasia of carotid artery. Methods: Forty-eight female New Zealand rabbits were randomly divided into four groups: control (balloon-induced carotid artery injury only); ovariectomy control (ovariectomy and carotid artery injury), oestrogen (ovariectomy, carotid artery injury and nilestriol, 5. mg/kg daily for 28 days), and isoflavone (ovariectomy, carotid artery injury and isoflavone 120. mg/kg daily for 28 days). The arterial wall thickness was assessed by coloured ultrasonography, and the oestrogen-' and oestrogen-{\~A}Ÿ receptors in the abdominal aorta were measured by Western blotting. Results: The medial layer thickness in the isoflavone group was less than in the ovariectomy control group (0.28 ± 0.03 vs. 0.35 ± 0.04. mm, p< 0.01), and the intimal/medial layer (I/. M) ratio is the isoflavone group was also less than in the ovariectomy control group (16.85 ± 3.79 vs. 48.94 ± 8.92, p< 0.01). There was no statistically significant difference in the medial layer thickness or I/. M ratio between the isoflavone and the oestrogen groups. The optical density of the oestrogen-' receptors in the isoflavone group (0.317 ± 0.002) was less than in the oestrogen (0.633 ± 0.002) or ovariectomy control group (0.590 ± 0.001, p< 0.01). The optical density of the oestrogen-{\~A}Ÿ receptors in the isoflavone group (1.350 ± 0.002) and the ovariectomy control group (1.2033 ± 0.002) was less than in the oestrogen group (1.7699 ± 0.003, p< 0.01). Conclusions: Isoflavone therapy in the ovariectomised rabbit model attenuated balloon catheter-induced intimal and medial layer hyperplasia in the carotid arteries. Down-regulation of the oestrogen-' receptors may be involved in the hyperplasia-preventative effect.",
keywords = "Open access version available, Hyperplasia, Isoflavone, Oestrogen receptors, Rabbits",
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Effect of isoflavone on balloon catheter-induced neointimal hyperplasia in rabbit carotid artery. / Zhang, G-Y.; Qiu, R.-F; Sun, Y.-C; Wang, Lexin.

In: Heart Lung and Circulation, Vol. 22, No. 2, 02.2013, p. 141-145.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of isoflavone on balloon catheter-induced neointimal hyperplasia in rabbit carotid artery

AU - Zhang, G-Y.

AU - Qiu, R.-F

AU - Sun, Y.-C

AU - Wang, Lexin

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = February, 2013; Journal title (773t) = Heart Lung and Circulation. ISSNs: 1443-9506;

PY - 2013/2

Y1 - 2013/2

N2 - Background: This study was designed to investigate the effects of phytoestrogen isoflavone on balloon catheter-induced hyperplasia of carotid artery. Methods: Forty-eight female New Zealand rabbits were randomly divided into four groups: control (balloon-induced carotid artery injury only); ovariectomy control (ovariectomy and carotid artery injury), oestrogen (ovariectomy, carotid artery injury and nilestriol, 5. mg/kg daily for 28 days), and isoflavone (ovariectomy, carotid artery injury and isoflavone 120. mg/kg daily for 28 days). The arterial wall thickness was assessed by coloured ultrasonography, and the oestrogen-' and oestrogen-ß receptors in the abdominal aorta were measured by Western blotting. Results: The medial layer thickness in the isoflavone group was less than in the ovariectomy control group (0.28 ± 0.03 vs. 0.35 ± 0.04. mm, p< 0.01), and the intimal/medial layer (I/. M) ratio is the isoflavone group was also less than in the ovariectomy control group (16.85 ± 3.79 vs. 48.94 ± 8.92, p< 0.01). There was no statistically significant difference in the medial layer thickness or I/. M ratio between the isoflavone and the oestrogen groups. The optical density of the oestrogen-' receptors in the isoflavone group (0.317 ± 0.002) was less than in the oestrogen (0.633 ± 0.002) or ovariectomy control group (0.590 ± 0.001, p< 0.01). The optical density of the oestrogen-ß receptors in the isoflavone group (1.350 ± 0.002) and the ovariectomy control group (1.2033 ± 0.002) was less than in the oestrogen group (1.7699 ± 0.003, p< 0.01). Conclusions: Isoflavone therapy in the ovariectomised rabbit model attenuated balloon catheter-induced intimal and medial layer hyperplasia in the carotid arteries. Down-regulation of the oestrogen-' receptors may be involved in the hyperplasia-preventative effect.

AB - Background: This study was designed to investigate the effects of phytoestrogen isoflavone on balloon catheter-induced hyperplasia of carotid artery. Methods: Forty-eight female New Zealand rabbits were randomly divided into four groups: control (balloon-induced carotid artery injury only); ovariectomy control (ovariectomy and carotid artery injury), oestrogen (ovariectomy, carotid artery injury and nilestriol, 5. mg/kg daily for 28 days), and isoflavone (ovariectomy, carotid artery injury and isoflavone 120. mg/kg daily for 28 days). The arterial wall thickness was assessed by coloured ultrasonography, and the oestrogen-' and oestrogen-ß receptors in the abdominal aorta were measured by Western blotting. Results: The medial layer thickness in the isoflavone group was less than in the ovariectomy control group (0.28 ± 0.03 vs. 0.35 ± 0.04. mm, p< 0.01), and the intimal/medial layer (I/. M) ratio is the isoflavone group was also less than in the ovariectomy control group (16.85 ± 3.79 vs. 48.94 ± 8.92, p< 0.01). There was no statistically significant difference in the medial layer thickness or I/. M ratio between the isoflavone and the oestrogen groups. The optical density of the oestrogen-' receptors in the isoflavone group (0.317 ± 0.002) was less than in the oestrogen (0.633 ± 0.002) or ovariectomy control group (0.590 ± 0.001, p< 0.01). The optical density of the oestrogen-ß receptors in the isoflavone group (1.350 ± 0.002) and the ovariectomy control group (1.2033 ± 0.002) was less than in the oestrogen group (1.7699 ± 0.003, p< 0.01). Conclusions: Isoflavone therapy in the ovariectomised rabbit model attenuated balloon catheter-induced intimal and medial layer hyperplasia in the carotid arteries. Down-regulation of the oestrogen-' receptors may be involved in the hyperplasia-preventative effect.

KW - Open access version available

KW - Hyperplasia

KW - Isoflavone

KW - Oestrogen receptors

KW - Rabbits

U2 - 10.1016/j.hlc.2012.07.010

DO - 10.1016/j.hlc.2012.07.010

M3 - Article

C2 - 23177646

VL - 22

SP - 141

EP - 145

JO - Heart Lung and Circulation

JF - Heart Lung and Circulation

SN - 1443-9506

IS - 2

ER -