Effect of local anesthesia on facial nerve blood flow and muscle action potential

Cheol Hee Choi, Sokcheon Pak, Chul Ho Jang

Research output: Contribution to journalArticle

Abstract

Background & objective: The effect of direct application of local lidocaine with epinephrine on the facial nerve (FN) has not been reported. The aim of this study is to assess the effects of 2% lidocaine with 1:100,000 epinephrine at clinically relevant concentrations in a rat FN model with respect to facial nerve blood flow (FNBF) and subsequent electrophysiological changes. Materials and methods: To assess the influence of drugs on FNBF and electrically evoked muscle action potential (EMAP), small pieces of gelfoam were soaked in PBS 100 μl (n = 5, control group), 50 μl (n = 5, treatment group A) and 100 μl (n = 5, group B) of 2% lidocaine with 1:100,000 epinephrine, and 50 μl (n = 5, group C) and 100 μl (n = 5, group D) of 2% lidocaine. After 5 min of stable recordings, we applied a 2% lidocaine with or without 1:100,000 epinephrine impregnated gelfoam over the main trunk of the facial nerve of rats for 30 min. After removing the applied gelfoam, FNBF and threshold of EMAP were measured separately in each group. Results: Compared to the control group, the treatment groups showed a significant reduction in FNBF in a dose-dependent manner. The maximal reductions in FNBF were observed in all treatment groups for a period after 10 min of the application. Synergistic reduction in FNBF was greater in groups A and B than in the lidocaine applied groups (C and D). The maximal increase in the EMAP threshold was observed immediately after the respective drug application in all groups. The greatest increase in the EMAP threshold was observed in group B. The increased EMAP threshold returned to the baseline value within 120 min in groups A and C. Conclusion: From these results, it can be considered that the topical application of lidocaine with epinephrine caused reduction in FNBF and elevation of EMAP threshold. These acute reductions in FNBF and elevations in the EMAP threshold were restored in a time-dependent manner.
Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume82
DOIs
Publication statusPublished - Mar 2016

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Facial Nerve
Local Anesthesia
Action Potentials
Lidocaine
Muscles
Epinephrine
Absorbable Gelatin Sponge
Control Groups
Pharmaceutical Preparations

Cite this

@article{21b222121e2349b0acc5ac3ade645d3a,
title = "Effect of local anesthesia on facial nerve blood flow and muscle action potential",
abstract = "Background & objective: The effect of direct application of local lidocaine with epinephrine on the facial nerve (FN) has not been reported. The aim of this study is to assess the effects of 2{\%} lidocaine with 1:100,000 epinephrine at clinically relevant concentrations in a rat FN model with respect to facial nerve blood flow (FNBF) and subsequent electrophysiological changes. Materials and methods: To assess the influence of drugs on FNBF and electrically evoked muscle action potential (EMAP), small pieces of gelfoam were soaked in PBS 100 μl (n = 5, control group), 50 μl (n = 5, treatment group A) and 100 μl (n = 5, group B) of 2{\%} lidocaine with 1:100,000 epinephrine, and 50 μl (n = 5, group C) and 100 μl (n = 5, group D) of 2{\%} lidocaine. After 5 min of stable recordings, we applied a 2{\%} lidocaine with or without 1:100,000 epinephrine impregnated gelfoam over the main trunk of the facial nerve of rats for 30 min. After removing the applied gelfoam, FNBF and threshold of EMAP were measured separately in each group. Results: Compared to the control group, the treatment groups showed a significant reduction in FNBF in a dose-dependent manner. The maximal reductions in FNBF were observed in all treatment groups for a period after 10 min of the application. Synergistic reduction in FNBF was greater in groups A and B than in the lidocaine applied groups (C and D). The maximal increase in the EMAP threshold was observed immediately after the respective drug application in all groups. The greatest increase in the EMAP threshold was observed in group B. The increased EMAP threshold returned to the baseline value within 120 min in groups A and C. Conclusion: From these results, it can be considered that the topical application of lidocaine with epinephrine caused reduction in FNBF and elevation of EMAP threshold. These acute reductions in FNBF and elevations in the EMAP threshold were restored in a time-dependent manner.",
keywords = "Action potential; Blood flow; Epinephrine; Facial nerve; Lidocaine; Topical",
author = "Choi, {Cheol Hee} and Sokcheon Pak and Jang, {Chul Ho}",
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Effect of local anesthesia on facial nerve blood flow and muscle action potential. / Choi, Cheol Hee; Pak, Sokcheon; Jang, Chul Ho.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 82, 03.2016, p. 43-46.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of local anesthesia on facial nerve blood flow and muscle action potential

AU - Choi, Cheol Hee

AU - Pak, Sokcheon

AU - Jang, Chul Ho

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = March; Journal title (773t) = International Journal of Pediatric Otorhinolaryngology. ISSNs: 0165-5876;

PY - 2016/3

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N2 - Background & objective: The effect of direct application of local lidocaine with epinephrine on the facial nerve (FN) has not been reported. The aim of this study is to assess the effects of 2% lidocaine with 1:100,000 epinephrine at clinically relevant concentrations in a rat FN model with respect to facial nerve blood flow (FNBF) and subsequent electrophysiological changes. Materials and methods: To assess the influence of drugs on FNBF and electrically evoked muscle action potential (EMAP), small pieces of gelfoam were soaked in PBS 100 μl (n = 5, control group), 50 μl (n = 5, treatment group A) and 100 μl (n = 5, group B) of 2% lidocaine with 1:100,000 epinephrine, and 50 μl (n = 5, group C) and 100 μl (n = 5, group D) of 2% lidocaine. After 5 min of stable recordings, we applied a 2% lidocaine with or without 1:100,000 epinephrine impregnated gelfoam over the main trunk of the facial nerve of rats for 30 min. After removing the applied gelfoam, FNBF and threshold of EMAP were measured separately in each group. Results: Compared to the control group, the treatment groups showed a significant reduction in FNBF in a dose-dependent manner. The maximal reductions in FNBF were observed in all treatment groups for a period after 10 min of the application. Synergistic reduction in FNBF was greater in groups A and B than in the lidocaine applied groups (C and D). The maximal increase in the EMAP threshold was observed immediately after the respective drug application in all groups. The greatest increase in the EMAP threshold was observed in group B. The increased EMAP threshold returned to the baseline value within 120 min in groups A and C. Conclusion: From these results, it can be considered that the topical application of lidocaine with epinephrine caused reduction in FNBF and elevation of EMAP threshold. These acute reductions in FNBF and elevations in the EMAP threshold were restored in a time-dependent manner.

AB - Background & objective: The effect of direct application of local lidocaine with epinephrine on the facial nerve (FN) has not been reported. The aim of this study is to assess the effects of 2% lidocaine with 1:100,000 epinephrine at clinically relevant concentrations in a rat FN model with respect to facial nerve blood flow (FNBF) and subsequent electrophysiological changes. Materials and methods: To assess the influence of drugs on FNBF and electrically evoked muscle action potential (EMAP), small pieces of gelfoam were soaked in PBS 100 μl (n = 5, control group), 50 μl (n = 5, treatment group A) and 100 μl (n = 5, group B) of 2% lidocaine with 1:100,000 epinephrine, and 50 μl (n = 5, group C) and 100 μl (n = 5, group D) of 2% lidocaine. After 5 min of stable recordings, we applied a 2% lidocaine with or without 1:100,000 epinephrine impregnated gelfoam over the main trunk of the facial nerve of rats for 30 min. After removing the applied gelfoam, FNBF and threshold of EMAP were measured separately in each group. Results: Compared to the control group, the treatment groups showed a significant reduction in FNBF in a dose-dependent manner. The maximal reductions in FNBF were observed in all treatment groups for a period after 10 min of the application. Synergistic reduction in FNBF was greater in groups A and B than in the lidocaine applied groups (C and D). The maximal increase in the EMAP threshold was observed immediately after the respective drug application in all groups. The greatest increase in the EMAP threshold was observed in group B. The increased EMAP threshold returned to the baseline value within 120 min in groups A and C. Conclusion: From these results, it can be considered that the topical application of lidocaine with epinephrine caused reduction in FNBF and elevation of EMAP threshold. These acute reductions in FNBF and elevations in the EMAP threshold were restored in a time-dependent manner.

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