Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs

M. Karayannopoulou, D. Psalla, G. Kazakos, Panayiotis Loukopoulos, N. Giannakas, I. Savvas, M. Kritsepi-Konstantinou, A. Chantes, L.G. Papazoglou

Research output: Contribution to journalArticle

Abstract

OBJECTIVES:Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.METHODS:Three 2x2 cm full-thickness skin defects were created bilaterally on the dorsolateral area of the trunk of six Beagle dogs. The wounds of one randomly selected side received PRP treatment, whereas the contralateral wounds were left untreated (controls). Wound healing was evaluated by planimetry, laser-Doppler flowmetry measurements of tissue perfusion, and histologically.RESULTS:The rate of wound healing did not differ significantly between the two groups. Tissue perfusion was significantly higher in the PRP-treated group (p = 0.008) compared to controls on day 10. Histological evaluation revealed a trend towards greater collagen production and a significantly better collagen orientation (p = 0.019) in PRP-treated wounds on day 20.CLINICAL SIGNIFICANCE:Locally injected autologous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.
Original languageEnglish
Article numberhttp://dx.doi.org/10.3415/VCOT-14-06-0088
Pages (from-to)172-178
Number of pages7
JournalVeterinary and Comparative Orthopaedics and Traumatology
Volume28
Issue number3
DOIs
Publication statusPublished - May 2015

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Platelet-Rich Plasma
tissue repair
skin (animal)
Wound Healing
animal injuries
Dogs
Skin
dogs
collagen
Collagen
Perfusion
Wounds and Injuries
Laser-Doppler Flowmetry
Beagle
growth factors
lasers
cytokines
Intercellular Signaling Peptides and Proteins
therapeutics
Cytokines

Cite this

Karayannopoulou, M., Psalla, D., Kazakos, G., Loukopoulos, P., Giannakas, N., Savvas, I., ... Papazoglou, L. G. (2015). Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. Veterinary and Comparative Orthopaedics and Traumatology, 28(3), 172-178. [http://dx.doi.org/10.3415/VCOT-14-06-0088]. https://doi.org/10.3415/VCOT-14-06-0088
Karayannopoulou, M. ; Psalla, D. ; Kazakos, G. ; Loukopoulos, Panayiotis ; Giannakas, N. ; Savvas, I. ; Kritsepi-Konstantinou, M. ; Chantes, A. ; Papazoglou, L.G. / Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. In: Veterinary and Comparative Orthopaedics and Traumatology. 2015 ; Vol. 28, No. 3. pp. 172-178.
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abstract = "OBJECTIVES:Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.METHODS:Three 2x2 cm full-thickness skin defects were created bilaterally on the dorsolateral area of the trunk of six Beagle dogs. The wounds of one randomly selected side received PRP treatment, whereas the contralateral wounds were left untreated (controls). Wound healing was evaluated by planimetry, laser-Doppler flowmetry measurements of tissue perfusion, and histologically.RESULTS:The rate of wound healing did not differ significantly between the two groups. Tissue perfusion was significantly higher in the PRP-treated group (p = 0.008) compared to controls on day 10. Histological evaluation revealed a trend towards greater collagen production and a significantly better collagen orientation (p = 0.019) in PRP-treated wounds on day 20.CLINICAL SIGNIFICANCE:Locally injected autologous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.",
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Karayannopoulou, M, Psalla, D, Kazakos, G, Loukopoulos, P, Giannakas, N, Savvas, I, Kritsepi-Konstantinou, M, Chantes, A & Papazoglou, LG 2015, 'Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs', Veterinary and Comparative Orthopaedics and Traumatology, vol. 28, no. 3, http://dx.doi.org/10.3415/VCOT-14-06-0088, pp. 172-178. https://doi.org/10.3415/VCOT-14-06-0088

Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. / Karayannopoulou, M.; Psalla, D.; Kazakos, G.; Loukopoulos, Panayiotis; Giannakas, N.; Savvas, I.; Kritsepi-Konstantinou, M.; Chantes, A.; Papazoglou, L.G.

In: Veterinary and Comparative Orthopaedics and Traumatology, Vol. 28, No. 3, http://dx.doi.org/10.3415/VCOT-14-06-0088, 05.2015, p. 172-178.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs

AU - Karayannopoulou, M.

AU - Psalla, D.

AU - Kazakos, G.

AU - Loukopoulos, Panayiotis

AU - Giannakas, N.

AU - Savvas, I.

AU - Kritsepi-Konstantinou, M.

AU - Chantes, A.

AU - Papazoglou, L.G.

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = May; Journal title (773t) = Veterinary and Comparative Orthopaedics and Traumatology. ISSNs: 0932-0814;

PY - 2015/5

Y1 - 2015/5

N2 - OBJECTIVES:Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.METHODS:Three 2x2 cm full-thickness skin defects were created bilaterally on the dorsolateral area of the trunk of six Beagle dogs. The wounds of one randomly selected side received PRP treatment, whereas the contralateral wounds were left untreated (controls). Wound healing was evaluated by planimetry, laser-Doppler flowmetry measurements of tissue perfusion, and histologically.RESULTS:The rate of wound healing did not differ significantly between the two groups. Tissue perfusion was significantly higher in the PRP-treated group (p = 0.008) compared to controls on day 10. Histological evaluation revealed a trend towards greater collagen production and a significantly better collagen orientation (p = 0.019) in PRP-treated wounds on day 20.CLINICAL SIGNIFICANCE:Locally injected autologous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.

AB - OBJECTIVES:Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.METHODS:Three 2x2 cm full-thickness skin defects were created bilaterally on the dorsolateral area of the trunk of six Beagle dogs. The wounds of one randomly selected side received PRP treatment, whereas the contralateral wounds were left untreated (controls). Wound healing was evaluated by planimetry, laser-Doppler flowmetry measurements of tissue perfusion, and histologically.RESULTS:The rate of wound healing did not differ significantly between the two groups. Tissue perfusion was significantly higher in the PRP-treated group (p = 0.008) compared to controls on day 10. Histological evaluation revealed a trend towards greater collagen production and a significantly better collagen orientation (p = 0.019) in PRP-treated wounds on day 20.CLINICAL SIGNIFICANCE:Locally injected autologous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.

KW - Dog

KW - Full-thickness skin defect

KW - Platelet-rich plasma

KW - Second intention wound healing

KW - Veterinary pathology

KW - Comparative pathology

KW - experimental surgery

KW - Skin disease

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Karayannopoulou M, Psalla D, Kazakos G, Loukopoulos P, Giannakas N, Savvas I et al. Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. Veterinary and Comparative Orthopaedics and Traumatology. 2015 May;28(3):172-178. http://dx.doi.org/10.3415/VCOT-14-06-0088. https://doi.org/10.3415/VCOT-14-06-0088