Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model

Jamie-Lee M Thompson; Daniel W D West; Thomas M Doering; Boris P Budiono; Sarah J Lessard; Lauren G Koch; Steven L Britton; Nuala M Byrne; Matthew A Brown; Kevin J Ashton; Vernon G Coffey

doi:10.1371/journal.pone.0261723

Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model

Jamie-Lee M Thompson, Daniel W D West, Thomas M Doering, Boris P Budiono, Sarah J Lessard, Lauren G Koch, Steven L Britton, Nuala M Byrne, Matthew A Brown, Kevin J Ashton, Vernon G Coffey

Dentistry and Medical Sciences

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Abstract

Skeletal muscle atrophy is a physiological response to disuse, aging, and disease. We compared changes in muscle mass and the transcriptome profile after short-term immobilization in a divergent model of high and low responders to endurance training to identify biological processes associated with the early atrophy response. Female rats selectively bred for high response to endurance training (HRT) and low response to endurance training (LRT; n = 6/group; generation 19) underwent 3 day hindlimb cast immobilization to compare atrophy of plantaris and soleus muscles with line-matched controls (n = 6/group). RNA sequencing was utilized to identify Gene Ontology Biological Processes with differential gene set enrichment. Aerobic training performed prior to the intervention showed HRT improved running distance (+60.6 ± 29.6%), while LRT were unchanged (-0.3 ± 13.3%). Soleus atrophy was greater in LRT vs. HRT (-9.0 ±8.8 vs. 6.2 ±8.2%; P

Original language	English
Article number	e0261723
Pages (from-to)	1-21
Number of pages	21
Journal	PLoS One
Volume	17
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0261723
Publication status	Published - Jan 2022

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10.1371/journal.pone.0261723

200501647 Published articleFinal published version, 2.8 MBLicence: CC BY

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Thompson, J.-L. M., West, D. W. D., Doering, T. M., Budiono, B. P., Lessard, S. J., Koch, L. G., Britton, S. L., Byrne, N. M., Brown, M. A., Ashton, K. J., & Coffey, V. G. (2022). Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model. PLoS One, 17(1 ), 1-21. Article e0261723. https://doi.org/10.1371/journal.pone.0261723

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abstract = "Skeletal muscle atrophy is a physiological response to disuse, aging, and disease. We compared changes in muscle mass and the transcriptome profile after short-term immobilization in a divergent model of high and low responders to endurance training to identify biological processes associated with the early atrophy response. Female rats selectively bred for high response to endurance training (HRT) and low response to endurance training (LRT; n = 6/group; generation 19) underwent 3 day hindlimb cast immobilization to compare atrophy of plantaris and soleus muscles with line-matched controls (n = 6/group). RNA sequencing was utilized to identify Gene Ontology Biological Processes with differential gene set enrichment. Aerobic training performed prior to the intervention showed HRT improved running distance (+60.6 ± 29.6%), while LRT were unchanged (-0.3 ± 13.3%). Soleus atrophy was greater in LRT vs. HRT (-9.0 ±8.8 vs. 6.2 ±8.2%; P",

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Thompson, J-LM, West, DWD, Doering, TM, Budiono, BP, Lessard, SJ, Koch, LG, Britton, SL, Byrne, NM, Brown, MA, Ashton, KJ & Coffey, VG 2022, 'Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model', PLoS One, vol. 17, no. 1 , e0261723, pp. 1-21. https://doi.org/10.1371/journal.pone.0261723

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AU - Koch, Lauren G

AU - Britton, Steven L

AU - Byrne, Nuala M

AU - Brown, Matthew A

AU - Ashton, Kevin J

AU - Coffey, Vernon G

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N2 - Skeletal muscle atrophy is a physiological response to disuse, aging, and disease. We compared changes in muscle mass and the transcriptome profile after short-term immobilization in a divergent model of high and low responders to endurance training to identify biological processes associated with the early atrophy response. Female rats selectively bred for high response to endurance training (HRT) and low response to endurance training (LRT; n = 6/group; generation 19) underwent 3 day hindlimb cast immobilization to compare atrophy of plantaris and soleus muscles with line-matched controls (n = 6/group). RNA sequencing was utilized to identify Gene Ontology Biological Processes with differential gene set enrichment. Aerobic training performed prior to the intervention showed HRT improved running distance (+60.6 ± 29.6%), while LRT were unchanged (-0.3 ± 13.3%). Soleus atrophy was greater in LRT vs. HRT (-9.0 ±8.8 vs. 6.2 ±8.2%; P

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Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model

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